Senior Scientist, In Vivo CAR-T Vector Engineering

Virtual Company; Gaithersburg (MD); On-site; USD 69,000 - 83,000; Full time

Job summary

The Fountain Group in Gaithersburg, MD is seeking a Senior Scientist to drive in vivo lentiviral vector platform development for CAR-T therapy. You will own experimental strategy, lead vector engineering, and manage functional assays for transduction and T cell design with minimal oversight.

Responsibilities include producing stable producer lines, validating vector strategies, and ensuring GMP-like documentation for audits.

Qualifications

• PhD or MS with industry experience in Cell Therapy, Virology, Immunology, Bioengineering, or related discipline.
• Extensive hands on experience in CAR T cell therapy development, ex vivo or in vivo.
• Deep expertise in lentiviral vector engineering, production, and QC.
• Advanced knowledge of primary T cell biology and functional assay development.
• Hands on proficiency in mammalian cell culture, including cancer cell lines and primary T cells.
• Advanced multicolor flow cytometry, including panel design, acquisition, analysis, and interpretation of transduction, phenotype, and function readouts.
• Proven ability to maintain exceptional documentation standards and scientific communication.

Responsibilities

• Generate and characterize stable producer cell lines, including clone screening, selection, and banking.
• Design, prioritize, and execute complex vector engineering and validation strategies for in vivo CAR delivery.
• Lead end to end lentiviral vector platform execution, including production, troubleshooting, optimization, and QC strategy.
• Own functional assessment strategy for vector potency and transduction, selecting appropriate assays and defining success criteria.
• Develop and execute flow cytometry-based assays to quantify transduction, target cell specificity, and CAR T phenotype and function.
• Ensure GMP grade like documentation standards, data traceability, and readiness for internal review or audit.
• Make independent technical decisions that impact project direction and experimental prioritizationIdentify knowledge gaps and proactively propose new approaches or technology improvements.
• Communicate complex findings clearly and persuasively to cross functional stakeholders.
• Role focus: Support CAR-T cell therapy research, specifically development of an in-vivo lentiviral vector (LVV) platform.
• Day-to-day work: Mostly lab-based, including lentivirus generation and QC, functional/titer analysis, primary-cell transduction, and T-cell/PBMC workflows.

Skills

CAR-T therapy

Lentiviral vector engineering

Primary T cell culture

Multicolor flow cytometry

In vivo LVV platform knowledge

Experimental design

Documentation and communication

Education

PhD or MS in relevant field

Tools

Mammalian cell culture

Job description

The Fountain Group in Gaithersburg, MD is seeking a Senior Scientist to drive in vivo lentiviral vector platform development for CAR-T therapy. You will own experimental strategy, lead vector engineering, and manage functional assays for transduction and T cell design with minimal oversight.

Responsibilities include producing stable producer lines, validating vector strategies, and ensuring GMP-like documentation for audits.