Protein Purification & Expression Scientist

Eurofins; West Point (PA); On-site; USD 70,000 - 90,000; Full time

Benefits offered by this job

Medical, dental, and vision benefits

Life and disability insurance

401(k) with company match

Paid vacation and holidays

Job summary

Eurofins USA PSS Insourcing Solutions in West Point, PA is seeking a protein expression and purification specialist to support small-scale screening and large-scale purification of recombinant proteins and nanoparticles. The role involves routine buffer prep, instrument maintenance, and record-keeping.

You will collaborate with the protein characterization group and other teams, applying aseptic techniques and ensuring SOP compliance. This position is full-time with standard work hours.

Qualifications

• BS with 3-5 years post-graduate work experience in protein expression and purification of recombinant proteins.
• Demonstrated expertise in research-scale affinity-based FPLC protein purification chromatography up to 100 mg.
• Knowledge of affinity, charge-based, and size-exclusion protein separation techniques; protein characterization techniques and protein-based assays.
• Aseptic technique and sterile cell culture in a biosafety cabinet; strong communication, independence, collaboration, and attention to SOPs.
• Microsoft Office proficiency and ability to work in a team.

Responsibilities

• Prepare buffers, maintain instruments, track work orders, sanitize chromatography columns, and keep detailed records.
• Provide hands-on support to the protein expression group as needed.
• Collaborate with protein characterization group and other teams.

Skills

Protein expression

FPLC purification

Chromatography

Aseptic technique

Cell culture

SDS-PAGE/Western blot

Data analysis

MS knowledge

Education

BS with 3-5 years post-graduate experience

MS with 1-2 years experience

Tools

Affinity chromatography

Size-exclusion chromatography

HPLC/UPLC

Bradford assay

UV-Vis spectroscopy

Job description

• Full-time

Company Description

Eurofins Scientific is an international life sciences company, providing a unique range of analytical testing services to clients across multiple industries, to make life and our environment safer, healthier and more sustainable. From the food you eat, to the water you drink, to the medicines you rely on, Eurofins laboratories work with the biggest companies in the world to ensure the products they supply are safe, their ingredients are authentic and labelling is accurate.

The Eurofins network of companies is the global leader in food, environment, pharmaceutical and cosmetic product testing and in agroscience Contract Research Organisation services. It is one of the market leaders in certain testing and laboratory services for genomics, discovery pharmacology, forensics, advanced material sciences and in the support of clinical studies, as well as having an emerging global presence in Contract Development and Manufacturing Organisations. It also has a rapidly developing presence in highly specialised and molecular clinical diagnostic testing and in-vitro diagnostic products.

In over 30 years, Eurofins has grown from one laboratory in Nantes, France to 58,000 staff across a decentralised and entrepreneurial network of 900 laboratories in over 54 countries. Eurofins companies offer a portfolio of over 200,000 analytical methods to evaluate the safety, identity, composition, authenticity, origin, traceability and purity of biological substances and products.

In 2021, Eurofins generated total revenues of EUR 6.72 billion, and has been among the best performing stocks in Europe over the past 20 years.

Job Description

The protein expression and purification team provides protein vaccine candidates and critical assay reagents to support target validation and identification during early discovery. The primary focus of this position will be supporting small-scale (30 mL culture) screening of protein antigens and large-scale (5 L culture) purification of recombinant proteins and nanoparticles.

Weekly tasks include:

• Preparation of buffers, maintenance of instruments, tracking of work orders, sanitization of chromatographic columns, detailed record-keeping, preparation of reports, and inventory maintenance.
• Hands-on support of the protein expression group is expected on occasion.
• The candidate will also collaborate with the protein characterization group, as well as other teams.

Qualifications

• BS with 3-5 years or more of post-graduate work experience, or MS with 1-2 years of graduate and post-graduate laboratory experience, in protein expression and purification of recombinant proteins.
• Demonstrated expertise in research-scale (up to 100 mg) affinity-based FPLC protein purification chromatography.
• Proven knowledge of the application of affinity, charge-based, and size-exclusion protein separation techniques.
  Proficiency in protein characterization techniques and protein-based assays such as Bradford protein quantitation, UV-Vis spectroscopy, SDS-PAGE, and Western blotting.
  A strong command of aseptic technique and sterile cell culture performed in a biosafety cabinet to maintain aseptic conditions is expected.
  The work requires effective communication skills, the ability to work independently, and the ability to collaborate with others. Meticulous attention to detail is required when following SOPs.
• A comprehensive understanding of Microsoft Office applications is a must.
• Authorization to work in the United States indefinitely without restrictions

Preferred Skills

• Knowledge of protein characterization by HPLC or UPLC. Proficiency in automated, tip-based chromatography systems and experience in recombinant protein expression.
• Experience with orthogonal protein characterization techniques (e.g., spectroscopy, MS).
• Proven track record in bacterial or mammalian cell culture, including cell passaging, cell counting, determination of cell density, and preparation of dilutions.

Additional Information

Position is full-time, Mon-Fri 8am-5pm. Candidates currently living within a commutable distance of West Point, PA are encouraged to apply.

• Excellent full time benefits including comprehensive medical coverage, dental, and vision options
• Life and disability insurance
• 401(k) with company match
• Paid vacation and holidays

Eurofins USA PSS Insourcing Solutions is a Disabled and Veteran Equal Employment Opportunity employer.