Scientist - Lentiviral Vector Development & CAR-T Cell Therapy

Rose International; South San Francisco (CA); In loco; USD 100.000 - 140.000; Tempo pieno

Descrizione del lavoro

Rose International is seeking a scientifically curious Scientist for a contingent position in cell therapy research. The role focuses on hands-on lentiviral vector production, vector characterization, and analytical assay development for in vivo and in vitro applications.

The candidate will generate materials for preclinical studies, collaborate across teams, and maintain rigorous records while adhering to biosafety standards. Excellent data interpretation and communication are essential.

Competenze

• Bachelors or Masters with at least 5 years of hands-on experience in biotechnology or cell therapy.
• PhD strongly preferred in immunology, virology, molecular biology, bioengineering, or related field.

Mansioni

• Execute and optimize research-scale lentiviral vector production workflows.
• Produce and characterize research-grade lentiviral vectors for in vitro evaluation and preclinical studies.
• Develop and optimize molecular and cell-based assays to assess vector quality, titer, gene delivery, and CAR expression.
• Perform primary human cell culture and engineering studies to evaluate delivery platforms.
• Apply flow cytometry to assess transduction efficiency, CAR expression, and cell phenotype.
• Collaborate with project teams to define material requirements and coordinate production timelines.
• Independently plan experiments and troubleshoot technical challenges with team input.
• Analyze and present findings at internal scientific meetings with clear summaries.

Conoscenze

Lentiviral vector production

Flow cytometry

Molecular assays

Cell culture (T cells)

Data interpretation

Formazione

Bachelors/Masters with 5 years industry experience

PhD in Immunology/Virology/ Molecular Biology/ Bioengineering/Cell Biology

Strumenti

FlowJo

GraphPad Prism

Benchling

Descrizione del lavoro

Required Education

• Bachelors/ Masters with 5 years industry experience.

Preferred Education

• PhD in Immunology, Virology, Molecular Biology, Bioengineering, Cell Biology, or a related discipline

Required Skills

• Hands-on experience with lentiviral vector production and characterization.
• Strong proficiency in aseptic mammalian cell culture and experience working with primary human immune cells, preferably T cells.
• Experience developing, optimizing, and troubleshooting molecular or cell-based analytical assays.
• Technical proficiency in flow cytometry and at least one quantitative molecular assay platform, such as qPCR or ddPCR.
• Demonstrated ability to independently design experiments, critically interpret data, and resolve technical challenges.
• Strong organizational, documentation, and communication skills, with the ability to manage concurrent experiments and collaborate effectively.

Preferred Skills

• Three or more years of relevant post-graduate research experience in biotechnology, pharmaceutical research, or an academic setting.
• Experience producing or characterizing gene delivery vectors for in vivo CAR T or related preclinical research programs.
• Experience with suspension-based lentiviral vector production, scale-up, or process development.
• Experience with CAR T-cell functional assays and multiparameter flow cytometry.
• Experience with nonviral delivery platforms, genome engineering, or next-generation sequencing.
• Familiarity with automation, liquid handling, and high-throughput analytical workflows.
• Proficiency with relevant analysis and documentation tools, such as FlowJo, GraphPad Prism, and Benchling.

Summary

The gRED Cell Therapy Department at Client is seeking a motivated and scientifically curious Scientist for a contingent position supporting in vivo cell therapy research. This hands‑on, laboratory‑based role will focus on research‑scale lentiviral vector production, vector characterization, and analytical assay development. The Scientist may also generate and characterize materials for use in preclinical in‑vivo studies conducted by collaborating teams. This position does not involve conducting animal studies. The successful candidate will bring strong experimental judgment, technical expertise in vector production and cell‑based analytics, and a collaborative approach to advancing research programs.

Responsibilities

• Execute and optimize research‑scale lentiviral vector production workflows, including approaches that support increased production capacity and transition to suspension culture.
• Produce and characterize research‑grade lentiviral vectors for in‑vitro evaluation and, as needed, to support preclinical in‑vivo studies.
• Develop, optimize, and execute molecular and cell‑based assays to assess vector quality, titer, gene delivery, CAR expression, and functional activity.
• Perform primary human cell culture and engineering studies to support evaluation of delivery platforms for in‑vivo cell therapy applications.
• Apply flow cytometry to assess transduction efficiency, CAR expression, and cell phenotype.
• Collaborate with project teams to define material requirements, coordinate production timelines, and provide supporting analytical data.
• Independently plan experiments, troubleshoot technical challenges, and interpret results in collaboration with project team members.
• Analyze and communicate findings through clear experimental summaries and presentations at internal scientific meetings.
• Maintain accurate and timely electronic laboratory records, document methods, and contribute to reproducible workflows.
• Support laboratory operations, including equipment onboarding and maintenance, and adhere to applicable biosafety, laboratory safety, and training requirements.
• Only those lawfully authorized to work in the designated country associated with the position will be considered.
• Please note that all Position start dates and duration are estimates and may be reduced or lengthened based upon a client’s business needs and requirements.

About Rose

• Founded in 1993
• Office Locations Across the U.S.
• 150+ Clients: Corporations and Government Agencies
• Employee Oriented Company
• Challenging Assignments Across the U.S.
• Continuous Professional Development

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