Research Assistant I - Cell Therapy

Baylor College of Medicine; Houston (TX); Vor Ort; USD 47.000 - 56.000; Vollzeit

Zusammenfassung

Baylor College of Medicine in Houston, TX is seeking a Research Assistant I to support a project in immune cell-based cancer immunotherapy under the supervision of Dr. Norihiro Watanabe.

The role focuses on preclinical work, including both in vitro and in vivo experiments. The position involves cell culture, molecular biology, flow cytometry, and data analysis, with duties across the research workflow in a collaborative environment.

Qualifikationen

• Bachelor's degree in a Basic Science or related field is required.
• At least two years of relevant laboratory experience is required.

Aufgaben

• Assist lab members to conduct preclinical work under PI guidance.
• Perform aseptic cell culture including primary cells and cell lines.
• Generate gene-modified therapeutic cells for preclinical work.
• Conduct in vitro functional assays such as flow cytometry and cytotoxicity assays.
• Perform gene expression analyses (Real-time PCR, RNA sequencing) and protein expression (flow cytometry, western blot).
• Generate virus constructs to enhance functionality of therapeutic immune cells.
• Collect and analyze data.
• Perform other duties as assigned.

Kenntnisse

Laboratory techniques

2 years lab experience

Ausbildung

Bachelor's degree in Basic Science

Jobbeschreibung

Job Title: Research Assistant I - Cell Therapy

Division: Center for Gene Therapy

Work Arrangement: Onsite only

Location: Houston, TX

Salary Range: $47,372 - $55731

FLSA Status: Nonexempt

Work Schedule: Monday – Friday, 8 a.m. – 5 p.m.

Summary

A Research Assistant I position will conduct research project focusing on immune cell-based cancer immunotherapy under supervision of Dr. Norihiro Watanabe. The person will assist and perform various preclinical works including both in vitro (cell culture, molecular biology, flow cytometry, etc) and in vivo (handling mouse, imaging, bleeding, injection) experiments.

Job Duties

• Assist lab member to conduct preclinical work under the guidance of PI as indicated below
• Perform aseptic cell culture including primary cells and cell lines
• Generate gene-modified therapeutic cells for preclinical work
• Conduct various in vitro functional assay such as cell surface phenotype (multi-color flow cytometry),
• Cytotoxicity assay (51Cr-release assay, Flow-based assay), cytokine detection (ELISA, Luminex assay), gene expression (Real-time PCR, RNA sequencing), and protein expression (flow cytometry, western blot)
• Generate virus constructs to enhance functionality of therapeutic immune cells (cloning, DNA/RNA isolation)
• Collect and analyze data
• Perform other job related duties as assigned

Minimum Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Two years of relevant experience.

Preferred Qualifications

• Bachelor's or Master's degree in a basic science, preferably Immunology, Cell Biology or Molecular Biology.
• Two years of laboratory work in industry or academia in a related field.
• Prior hands-on experience with cell culture, DNA/RNA processing, molecular cloning, gene transduction, flow cytometry, and handling mice.

Work Authorization Requirement:

This position is not eligible for visa sponsorship.Candidates must be legally authorized to work in the United States at the time of application and throughout the duration of employment.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

Baylor College of Medicine fosters diversity among its students, trainees, faculty, and staff as a prerequisite to accomplishing our institutional mission and setting standards for excellence in training healthcare providers and biomedical scientists, promoting scientific innovation, and providing patient-centered care. Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.