Protein Science Associate Scientist - Antibody Discovery

Alloy Therapeutics; Waltham (MA); On-site; USD 93,000 - 110,000; Full time

Benefits offered by this job

Unlimited PTO (paid time off)

Education stipend for ongoing learning

Wellness and EAP access

Job summary

Alloy Therapeutics in Waltham, MA, is seeking a hands-on protein science professional to support antibody discovery programs through transfection, DNA cloning, and purification activities. You will contribute to high-throughput and large-scale expression, with emphasis on quality, speed, and collaboration.

The role requires experience with mammalian expression systems, transfection optimization, and recombinant DNA techniques, with a focus on delivering robust workflows in a fast-paced,

Qualifications

• MS with 2+ years or BS with 4+ years hands-on research in molecular biology or related discipline.
• Degree in molecular biology, cell biology, biochemistry, immunology, or related field.
• Experience with transient expression of antibodies using mammalian systems (CHO preferred).
• Knowledge of transfection methods (PEI, lipofection, electroporation) and optimization of transfection efficiency.
• Proficiency in high-density suspension cell culture management and scale-up strategies.
• Hands-on experience with recombinant DNA techniques including PCR, cloning, restriction digestion, gel analysis, and plasmid purification.
• Experience with monoclonal, bispecific, and multi-specific constructs.
• Strong organizational skills to manage multiple projects and maintain compliance.

Responsibilities

• Perform small- to large-scale transient transfections in mammalian cell lines (e.g. CHO) to drive high yield biologics production.
• Independently manage multiple cell culture lines maintaining proper tissue culture techniques.
• Systematically evaluate and optimize transfection efficiency, cell viability, and expression protocols across scales to improve throughput and yield.
• Execute recombinant DNA workflows, including PCR, Gibson assembly, and plasmid scale‑up.
• Contribute to antibody construct engineering to support production of multi-specific candidates.
• Support protein expression and purification of biologics using AKTA chromatography systems.
• Conduct regular QC on biologics including analytics like aSEC, CE-SDS, SDS-PAGE, and LAL as needed.
• Analyze and track deliverables in ELN; coordinate handoffs across teams for workflow continuity.
• Present experimental data and findings in team meetings; ensure timely execution aligned with priorities.
• Maintain laboratory organization and safety compliance.

Skills

Molecular biology

Mammalian transfection

Recombinant DNA techniques

Cloning & PCR

Cell culture (CHO)

Documentation & compliance

Education

MS with 2+ years hands-on research

BS with 4+ years hands-on research

Tools

AKTA chromatography

Vi-CELL

LIMS familiarity

Job description

Alloy Therapeutics in Waltham, MA, is seeking a hands-on protein science professional to support antibody discovery programs through transfection, DNA cloning, and purification activities. You will contribute to high-throughput and large-scale expression, with emphasis on quality, speed, and collaboration.

The role requires experience with mammalian expression systems, transfection optimization, and recombinant DNA techniques, with a focus on delivering robust workflows in a fast-paced,