Optogenetic Cell-Line Development Researcher

DutchTechX; Emeryville (CA); Sur place; USD 73 000 - 118 000; Plein temps

Avantages offerts par ce poste

Equity package

Retirement plan

Generous PTO

Medical, dental, and vision insurance

Life and disability insurance

Career coaching

Mental health support

Free team lunches

Snacks

Team events

Résumé du poste

Prolific Machines in Emeryville, CA is seeking a Research Associate to advance mammalian cell line development and protein production using light-controlled systems. You will generate and characterize cell lines, perform transfections, and support downstream readouts in a fast-paced startup environment.

Collaborate across disciplines, design experiments, and maintain thorough documentation. You will visualize data and present findings in team meetings, with on-site work and growth opportunities

Qualifications

• Bachelor’s degree in biology or related field with mammalian cell culture experience.
• Proficiency in sterile technique and transfection.
• Experience with cell line development and clonal isolation.
• Familiar with readouts: cell counting, metabolite measurement, Octet BLI, flow cytometry.
• Ability to design experiments and troubleshoot analytical techniques.

Responsabilités

• Own workflows using mammalian cell culture with HEK/CHO lines.
• Generate stable cell lines via transfection and selection; manage multiple lines.
• Handle protein production workflows at small scales (deep wells, flasks).
• Perform readouts (cell counting, metabolite, Octet BLI, flow cytometry).
• Conduct single cell isolation using FACS or related methods.
• Execute plasmid cloning and ddPCR; document experiments clearly.
• Analyze data and present findings in team meetings.

Connaissances

Mammalian culture

Transfection

Molecular cloning

Flow cytometry

Data analysis

Experiment design

Formation

Bachelor's in Biology/related

Outils

Ambr bioreactor

CellecA counter

FACS

Nucleocounter

Description du poste

Prolific Machines in Emeryville, CA is seeking a Research Associate to advance mammalian cell line development and protein production using light-controlled systems. You will generate and characterize cell lines, perform transfections, and support downstream readouts in a fast-paced startup environment.

Collaborate across disciplines, design experiments, and maintain thorough documentation. You will visualize data and present findings in team meetings, with on-site work and growth opportunities