Mammalian Cell Line Development Research Associate

Prolific Machines; Emeryville (CA); Sur place; USD 73 000 - 118 000; Plein temps

Avantages offerts par ce poste

Equity package

Retirement plan with company contrib.

Flexible PTO with minimums

Medical, dental, and vision insurance

Life and disability insurance

Career coaching

Mental health support

Free team lunches everyday

Great snacks

Social events

Résumé du poste

Prolific Machines in Emeryville, CA is seeking a motivated Research Associate to advance mammalian cell line development and protein production using light-controlled expression. You will generate and characterize cell lines to support internal research and customer programs, collaborating across disciplines in a fast-paced startup environment.

The role focuses on transfection, stable cell line generation, and downstream readouts, with hands-on lab work and meticulous documentation.

Qualifications

• Bachelor’s degree in Biology, Bioengineering, Biotechnology, or related field.
• Experience in mammalian cell culture, sterile technique, transfection, and clone isolation.
• Experience with readouts such as cell counting, metabolite measurements, Octet BLI, and flow cytometry.
• Ability to develop and troubleshoot experimental plans and work independently.
• Strong organizational and data analysis skills.

Responsabilités

• Own workflows using mammalian cell culture with HEK and CHO cells.
• Generate stable cell lines via transfection and selection; own multiple cell lines.
• Manage protein production workflows from deep-well plates to small bioreactors.
• Perform readouts: Celleca, metabolite measurements, Octet BLI, flow cytometry.
• Execute single-cell isolation using FACS or Beacon.
• Conduct plasmid cloning and molecular biology (PCR, ddPCR).
• Document experiments and present data in team meetings.

Connaissances

Mammalian cell culture

Transfection

Clone isolation

Flow cytometry

Cell line development

Experimental design

Formation

Bachelor’s degree in Biology/Bioengineering/Biotechnology

Description du poste

Prolific Machines in Emeryville, CA is seeking a motivated Research Associate to advance mammalian cell line development and protein production using light-controlled expression. You will generate and characterize cell lines to support internal research and customer programs, collaborating across disciplines in a fast-paced startup environment.

The role focuses on transfection, stable cell line generation, and downstream readouts, with hands-on lab work and meticulous documentation.