Metabolite Profiling & Biotransformation Lead

Alnylam Pharmaceuticals; Cambridge (MA); On-site; USD 180,000 - 240,000; Full time

Job summary

Alnylam Pharmaceuticals in Cambridge, MA seeks a Principal Scientist, Biotransformation & Metabolite Profiling to lead drug metabolism characterization across discovery and development programs.

You will develop integrated metabolite profiling strategies using LC-HRMS/MS, mentor staff, collaborate with DMPK and cross-functional teams, and drive interpretation of metabolism data to inform safety and efficacy.

Qualifications

• PhD in analytical chemistry or related field with 6+ years of industry research.
• Extensive hands-on experience in drug metabolism, metabolite profiling, and metabolite identification for relevant modalities.
• Strong expertise in LC-MS/MS and high-resolution mass spectrometry.
• Demonstrated ability to elucidate metabolite structures and biotransformation pathways.
• Strong understanding of drug metabolism, PK, tissue distribution.
• Track record of publications or regulatory white papers.
• Detail-oriented with ability to manage multiple projects in a fast-paced environment.
• Excellent verbal and written communication skills.

Responsibilities

• Lead metabolite profiling and structural identification in plasma, urine, bile, tissues, and in vitro matrices.
• Support multiple therapeutic modalities (small molecules, peptides, RNAi, conjugates).
• Apply LC-HRMS/MS for metabolite detection and relative quantitation.
• Operate metabolite profiling software (e.g., CD, BioPharma Finder, ProMass, Mass MetaSite).
• Design biotransformation strategies using hepatocytes and other models.
• Characterize metabolic pathways and pharmacologically relevant metabolites.
• Support PK/PD analyses as needed; relate in vitro to in vivo metabolism.
• Collaborate with cross-functional teams and BA representatives; prepare IND-supporting reports.

Skills

Metabolite profiling

LC-MS/MS

Biotransformation

MS data analysis

Team leadership

Scientific communication

Education

PhD in analytical chemistry

Tools

Thermo Compound Discoverer

BioPharma Finder

ProMass

Mass MetaSite

Job description

Alnylam Pharmaceuticals in Cambridge, MA seeks a Principal Scientist, Biotransformation & Metabolite Profiling to lead drug metabolism characterization across discovery and development programs.

You will develop integrated metabolite profiling strategies using LC-HRMS/MS, mentor staff, collaborate with DMPK and cross-functional teams, and drive interpretation of metabolism data to inform safety and efficacy.