Lead Multicolor Flow Cytometry Panel Architect

Surf Search Inc.; South San Francisco (CA); Presencial; USD 120.000 - 180.000; Jornada completa

Descripción de la vacante

Surf Search Inc. seeks an experienced Flow Cytometry Scientist to design and optimize complex multicolor flow cytometry panels from scratch.

You will lead experimental design, fluorochrome selection, and spectral compensation to ensure high-quality data, with emphasis on robust panel validation and reproducibility. The role requires a PhD in Immunology or related field (or equivalent hands-on experience) and extensive experience in high-parameter cytometry.

Formación

• PhD or equivalent with extensive hands-on experience in multicolor flow cytometry.
• Experience designing complex panels from scratch beyond routine assays.
• Expertise in fluorochrome selection, titration, compensation, and spectral overlap handling.

Responsabilidades

• Design and develop high-parameter multicolor flow cytometry panels from scratch.
• Optimize antibody clones, fluorochromes, titration, compensation, and spectral unmixing to maximize data quality.
• Troubleshoot panels for spectral overlap, nonspecific binding, autofluorescence, and low signal resolution.
• Develop staining protocols, controls, and gating strategies for complex populations.
• Analyze and interpret flow cytometry data using FlowJo, FCS Express, or similar software.
• Document and validate optimized panels and protocols to ensure reproducibility.
• Collaborate with cross-functional teams to develop flow cytometry solutions for complex research questions.

Conocimientos

Multicolor flow cytometry

Panel design and optimization

Fluorochrome selection

Antibody titration

Spectral overlap compensation

Gating strategies

Data analysis (FlowJo/FCS Express)

Educación

PhD in Immunology / related field

Herramientas

FlowJo

FCS Express

Descripción del empleo

Surf Search Inc. seeks an experienced Flow Cytometry Scientist to design and optimize complex multicolor flow cytometry panels from scratch.

You will lead experimental design, fluorochrome selection, and spectral compensation to ensure high-quality data, with emphasis on robust panel validation and reproducibility. The role requires a PhD in Immunology or related field (or equivalent hands-on experience) and extensive experience in high-parameter cytometry.