Junior Lab Specialist: Lipid Biology & Protein Interactions

University of California San Francisco; San Francisco (CA); On-site; USD 57,000 - 91,000; Full time

Job summary

University of California San Francisco seeks a Junior/Assistant/Associate Specialist for the Jura lab to investigate molecular mechanisms regulating cellular lipid homeostasis, focusing on sphingolipid and cholesterol biology. Responsibilities include producing lipid-binding proteins, characterizing protein-lipid interactions, and analyzing lipid mass spectrometry data.

The role also covers mammalian cell culture, immunofluorescence-based localization analyses, flow cytometry, and laboratory

Qualifications

• Specialists appointed at the Junior rank must possess a Baccalaureate degree (or equivalent degree) or at least four years of research experience.
• Specialists appointed at the Assistant rank must possess a Master’s degree or a baccalaureate degree with 3 or more years of research experience.
• Specialist appointed at the Associate rank must possess a Master’s degree (or equivalent degree) or five to ten years of experience in the relevant specialization.
• Demonstrated expertise in the expression and purification of CNPY/SAPLIP-family lipid-binding proteins and bacterially expressed fluorescent lipid-binding probes; fluorescence polarization and in vitro lipid-binding assays; lipid mass spectrometry data analysis; mammalian cell culture; quantitative immunofluorescence-based analysis of protein localization and trafficking; and flow cytometry.
• Expert knowledge of sphingolipid and cholesterol biosynthesis, trafficking, and cellular homeostasis is required.
• Experience managing laboratory operations, including purchasing, shipping and receiving, EH&S compliance, and personnel safety training, is also required.

Responsibilities

• Produce and purify lipid-binding proteins and biosensors as described.
• Characterize protein-lipid interactions using fluorescence polarization and in vitro assays.
• Analyze lipid mass spectrometry data and interpret results.
• Maintain mammalian cell culture and perform quantitative immunofluorescence analyses.
• Perform flow cytometry data collection and analysis.
• Manage lab operations including purchasing, shipping/receiving, EH&S compliance, and training.

Skills

Protein expression/purification

Fluorescence lipid-binding probes

Lipid mass spectrometry data analysis

Mammalian cell culture

Immunofluorescence trafficking

Flow cytometry

Education

Baccalaureate degree

Master’s degree

Tools

Fluorescence polarization

Lipid mass spectrometry

Mammalian cell culture

Job description

University of California San Francisco seeks a Junior/Assistant/Associate Specialist for the Jura lab to investigate molecular mechanisms regulating cellular lipid homeostasis, focusing on sphingolipid and cholesterol biology. Responsibilities include producing lipid-binding proteins, characterizing protein-lipid interactions, and analyzing lipid mass spectrometry data.

The role also covers mammalian cell culture, immunofluorescence-based localization analyses, flow cytometry, and laboratory