Lipid Biology Research Specialist - Protein Purification

University of California, San Francisco; San Francisco (CA); On-site; USD 57,000 - 91,000; Full time

Job summary

The University of California, San Francisco invites applications for a Junior/Assistant/Associate Specialist in the Jura lab to study cellular lipid homeostasis, focusing on sphingolipid and cholesterol biology. The position involves production and purification of lipid-binding proteins and fluorescent probes, plus characterization of protein–lipid interactions.

The role also covers mammalian cell culture, immunofluorescence analyses, flow cytometry, and lipid mass spectrometry data

Qualifications

• Requires Bachelor’s degree or equivalent or 4+ years of research experience.
• Assistant rank requires Master’s degree or a BA with 3+ years of research experience.
• Associate rank requires Master’s degree or 5–10 years in the relevant specialization.
• Demonstrated expertise in expression and purification of CNPY/SAPLIP-family lipid-binding proteins and probes; fluorescence polarization and in vitro lipid-binding assays; lipid MS data analysis; mammalian cell culture; quantitative immunofluorescence; and flow cytometry.
• Expert knowledge of sphingolipid and cholesterol biosynthesis, trafficking, and cellular homeostasis.
• Experience managing laboratory operations, including purchasing, shipping/receiving, EH&S compliance, and personnel safety training.

Responsibilities

• Produce and purify lipid-binding proteins (CNPY/SAPLIP family) and fluorescent probes.
• Characterize protein–lipid interactions using fluorescence polarization and in vitro lipid-binding assays.
• Analyze lipid mass spectrometry data.
• Perform mammalian cell culture and quantitative immunofluorescence analysis of protein localization and trafficking.
• Collect and analyze flow cytometry data.
• Manage laboratory operations, including purchasing, shipping/receiving, EH&S compliance, and safety training.

Skills

Lipid protein expression & purif.

Fluorescence polarization

Lipid mass spectrometry data analysis

Mammalian cell culture

Immunofluorescence analysis

Flow cytometry

Lipid biosynthesis & homeostasis

Lab operations management

Protein–lipid interactions

Education

Bachelor’s degree or equivalent

Master’s degree

Job description

The University of California, San Francisco invites applications for a Junior/Assistant/Associate Specialist in the Jura lab to study cellular lipid homeostasis, focusing on sphingolipid and cholesterol biology. The position involves production and purification of lipid-binding proteins and fluorescent probes, plus characterization of protein–lipid interactions.

The role also covers mammalian cell culture, immunofluorescence analyses, flow cytometry, and lipid mass spectrometry data