Junior, Assistant, of Associate Specialist – Jura lab

University of California, San Francisco; San Francisco (CA); On-site; USD 57,000 - 91,000; Full time

Job summary

The University of California, San Francisco invites applications for a Junior/Assistant/Associate Specialist in the Jura lab to study cellular lipid homeostasis, focusing on sphingolipid and cholesterol biology. The position involves production and purification of lipid-binding proteins and fluorescent probes, plus characterization of protein–lipid interactions.

The role also covers mammalian cell culture, immunofluorescence analyses, flow cytometry, and lipid mass spectrometry data

Qualifications

• Requires Bachelor’s degree or equivalent or 4+ years of research experience.
• Assistant rank requires Master’s degree or a BA with 3+ years of research experience.
• Associate rank requires Master’s degree or 5–10 years in the relevant specialization.
• Demonstrated expertise in expression and purification of CNPY/SAPLIP-family lipid-binding proteins and probes; fluorescence polarization and in vitro lipid-binding assays; lipid MS data analysis; mammalian cell culture; quantitative immunofluorescence; and flow cytometry.
• Expert knowledge of sphingolipid and cholesterol biosynthesis, trafficking, and cellular homeostasis.
• Experience managing laboratory operations, including purchasing, shipping/receiving, EH&S compliance, and personnel safety training.

Responsibilities

• Produce and purify lipid-binding proteins (CNPY/SAPLIP family) and fluorescent probes.
• Characterize protein–lipid interactions using fluorescence polarization and in vitro lipid-binding assays.
• Analyze lipid mass spectrometry data.
• Perform mammalian cell culture and quantitative immunofluorescence analysis of protein localization and trafficking.
• Collect and analyze flow cytometry data.
• Manage laboratory operations, including purchasing, shipping/receiving, EH&S compliance, and safety training.

Skills

Lipid protein expression & purif.

Fluorescence polarization

Lipid mass spectrometry data analysis

Mammalian cell culture

Immunofluorescence analysis

Flow cytometry

Lipid biosynthesis & homeostasis

Lab operations management

Protein–lipid interactions

Education

Bachelor’s degree or equivalent

Master’s degree

Job description

Position Description

Junior, Assistant, of Associate Specialist – Jura lab

The position will focus on investigating the molecular mechanisms that regulate cellular lipid homeostasis, with particular emphasis on sphingolipid and cholesterol biology. Duties will include production and purification of lipid-binding proteins, including members of the CNPY/SAPLIP family and bacterially expressed fluorescent lipid-binding probes; characterization of protein–lipid interactions using biophysical and biochemical approaches, including fluorescence polarization and in vitro lipid-binding assays; and analysis and interpretation of lipid mass spectrometry data. The position will also involve mammalian cell culture, quantitative immunofluorescence-based analysis of protein localization and intracellular trafficking, and flow cytometry data collection and analysis. The work requires extensive expertise in the biosynthesis, trafficking, and cellular homeostasis of sphingolipids and cholesterol, as well as their regulation by lipid-binding proteins.

In addition, the position will include laboratory management responsibilities, including ordering and maintaining laboratory supplies, coordinating shipping and receiving, ensuring compliance with Environmental Health and Safety (EH&S) requirements, and maintaining records to ensure that all laboratory personnel have completed required safety and compliance training.

Required Qualifications

• Specialists appointed at the Junior rank must possess a Baccalaureate degree (or equivalent degree) or at least four years of research experience.


• Specialists appointed at the Assistant rank must possess a Master’s degree or a baccalaureate degree with 3 or more years of research experience.


• Specialist appointed at the Associate rank must possess a Master’s degree (or equivalent degree) or five to ten years of experience in the relevant specialization.


• Demonstrated expertise in the expression and purification of CNPY/SAPLIP-family lipid-binding proteins and bacterially expressed fluorescent lipid-binding probes; fluorescence polarization and in vitro lipid-binding assays; lipid mass spectrometry data analysis; mammalian cell culture; quantitative immunofluorescence-based analysis of protein localization and trafficking; and flow cytometry.


• Expert knowledge of sphingolipid and cholesterol biosynthesis, trafficking, and cellular homeostasis is required.


• Experience managing laboratory operations, including purchasing, shipping and receiving, EH&S compliance, and personnel safety training, is also required.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $57,000 - $91,100.

Document Requirements

• Curriculum Vitae - CV must clearly list current and/or pending qualifications (e.g. board eligibility/certification, medical licensure, etc.).


• Cover Letter (Optional)


• Statement of Research (Optional)


• Statement of Teaching (Optional)

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.

UCSF is committed to welcoming and serving all people, honoring the dignity of every individual without preference or prejudice, in support of its public mission and in alignment with our PRIDE values and Principles of Community.

As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.

• “Misconduct” means any violation of the policies or laws governing conduct at the applicant’s previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, or discrimination, as defined by the employer.


• UC Sexual Violence and Sexual Harassment Policy


• UC Anti-Discrimination Policy


• APM - 035: Affi Ofitive Action and Nondiscrimination in Employment

San Francisco, CA