Junior Lipid Biology Research Specialist

University of California - San Francisco; San Francisco (CA); On-site; USD 57,000 - 91,000; Full time

Job summary

University of California - San Francisco is seeking a Junior/Assistant/Associate Specialist in the Jura lab to study molecular mechanisms regulating lipid homeostasis, emphasizing sphingolipid and cholesterol biology. Responsibilities include producing and purifying CNPY/SAPLIP-family proteins and probes, and analyzing protein-lipid interactions using various assays.

The role covers mammalian cell culture, quantitative immunofluorescence analysis, flow cytometry, and lab operations such as

Qualifications

• Baccalaureate degree (or equivalent) or at least 4 years of research experience.
• Master's degree or baccalaureate degree with 3+ years of research experience.
• Master's degree (or equivalent) or five to ten years of experience for Associate rank.
• Expertize in expression/purification of CNPY/SAPLIP-family lipid-binding proteins and related probes.
• Experience with fluorescence polarization, lipid mass spectrometry data analysis, and flow cytometry.
• Knowledge of sphingolipid and cholesterol biosynthesis, trafficking, and homeostasis.

Responsibilities

• Produce and purify lipid-binding proteins and probes.
• Characterize protein-lipid interactions using biophysical and biochemical methods.
• Conduct lipid mass spectrometry data analysis.
• Perform mammalian cell culture and quantitative immunofluorescence for protein localization.
• Analyze intracellular trafficking and flow cytometry data.
• Manage laboratory operations including purchasing, shipping, EH&S compliance, and safety training.

Skills

Protein expression

Protein purification

Fluorescence polarization

Lipid-binding assays

Lipid mass spectrometry

Mammalian cell culture

Immunofluorescence

Flow cytometry

Education

Master's degree

Baccalaureate degree + 3+ years research

Job description

University of California - San Francisco is seeking a Junior/Assistant/Associate Specialist in the Jura lab to study molecular mechanisms regulating lipid homeostasis, emphasizing sphingolipid and cholesterol biology. Responsibilities include producing and purifying CNPY/SAPLIP-family proteins and probes, and analyzing protein-lipid interactions using various assays.

The role covers mammalian cell culture, quantitative immunofluorescence analysis, flow cytometry, and lab operations such as