Immunology Discovery Scientist - Cell-Based Assays

100 Eli Lilly and Company; Indianapolis (IN); On-site; USD 66,000 - 172,000; Full time

Job summary

Eli Lilly and Company in Indianapolis, IN, is seeking a scientist to design and validate immunology cell-based assays supporting preclinical data for novel therapeutics. The role focuses on primary lymphocytes from human, monkey, and mouse, with emphasis on multi-parameter flow cytometry and downstream analyses.

You will work under a Ph.D. senior scientist in a collaborative team, performing independent experiments and maintaining meticulous e-notebook records to ensure high-quality data for

Qualifications

• B.S or M.S. in biology, molecular and cellular immunology or a related biological area with 2-3 years of work experience in an academic institute or pharmaceutical industry.
• Research experience in immunology and molecular biology.
• Previous experience in purification and handling primary human lymphocytes (and other species) is highly desirable.

Responsibilities

• Design, develop and validate immunology cell-based functional assays to deliver preclinical data for novel therapeutics.
• Develop immune functional cell-based assays using primary lymphocytes and cytokine assays.
• Screen and analyze data for protein or small molecule drugs in cell-based assays.
• Isolate, purify and culture human, monkey, and mouse lymphocytes for in vitro assays.
• Develop cellular activity assays including western blot, qPCR, intracellular staining, and phospho-flow cytometry.
• Investigate cellular signal transduction including cytokine/receptor signaling.
• Independently design and execute experiments, troubleshoot, analyze and present data.
• Maintain good lab practices and e-notebook records.

Skills

Immunology concepts

Cell culture

Flow cytometry

qPCR

ELISA / MSD multiplex

siRNA knockdown

Data analysis

Sterile technique

Education

B.S. or M.S. in biology/immunology

Tools

Western blot

qPCR machine

Flow cytometer

Job description

Eli Lilly and Company in Indianapolis, IN, is seeking a scientist to design and validate immunology cell-based assays supporting preclinical data for novel therapeutics. The role focuses on primary lymphocytes from human, monkey, and mouse, with emphasis on multi-parameter flow cytometry and downstream analyses.

You will work under a Ph.D. senior scientist in a collaborative team, performing independent experiments and maintaining meticulous e-notebook records to ensure high-quality data for