CRISPR & iPSC Research Technician II

Partner's Healthcare; Cambridge (MA); On-site; USD 33,000 - 54,000; Full time

Job summary

Mass General Brigham Incorporated in Cambridge, MA, seeks a Research Technician II for the Gene Editing Core to support mammalian cell culture, genome editing, iPSC reprogramming, and NGS workflows in a fast-paced core facility.

The role requires a Bachelor’s degree and 1–2 years of hands-on lab experience; familiarity with CRISPR-based editing and sequencing QC is preferred. On-site position at 65 Landsdowne Street with 40 hours weekly and competitive pay.

Qualifications

• Bachelor's degree required.
• Minimum of 1-2 years of hands-on research laboratory experience, including mammalian cell culture and molecular biology techniques.

Responsibilities

• Support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and lentiviral vector production workflows.
• Maintain and culture mammalian cell lines, including human iPSCs and primary cells, using aseptic technique.
• Perform routine molecular biology procedures, including DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid preparation.
• Support CRISPR-based genome-editing workflows, including reagent preparation, transfection/nucleofection, single-cell clone isolation and expansion, and genotyping of edited clones.
• Support next-generation sequencing (NGS) workflows, including amplicon/library preparation, sample quality control, and preparation of samples for sequencing.
• Perform cell characterization and quality-control assays, including immunofluorescence staining, microscopy, and other established assays.
• Maintain accurate experimental records, sample inventories, and project documentation according to established core SOPs.
• Perform routine data collection, organization, and basic analysis; communicate experimental results to core staff.
• Assist with laboratory inventory, reagent preparation, equipment maintenance, and general core operations.
• Follow established SOPs, biosafety requirements, and laboratory quality practices.
• Assist with troubleshooting and optimization of established gene-editing and molecular biology workflows under appropriate supervision.
• Participate in team meetings, training activities, and continuous workflow improvement.

Skills

Mammalian cell culture

Molecular biology

CRISPR-based editing

qPCR

Data analysis

Education

Bachelor's degree

Tools

Lentiviral vector production

NGS library prep

PCR

Gel electrophoresis

Job description

Mass General Brigham Incorporated in Cambridge, MA, seeks a Research Technician II for the Gene Editing Core to support mammalian cell culture, genome editing, iPSC reprogramming, and NGS workflows in a fast-paced core facility.

The role requires a Bachelor’s degree and 1–2 years of hands-on lab experience; familiarity with CRISPR-based editing and sequencing QC is preferred. On-site position at 65 Landsdowne Street with 40 hours weekly and competitive pay.