Research Fellow (Colorectal Cancer Study)

Singapore General Hospital; Singapore; On-site; SGD 60,000 - 90,000; Full time

Job summary

Singapore General Hospital Pte Ltd. is seeking a highly skilled researcher to plan and conduct experiments for a study on combination therapy in colorectal cancer.

You will work closely with the PI to design and troubleshoot experiments, establish cancer cell models and organoids, and evaluate treatment responses using flow cytometry, IHC, and related assays. You will coordinate sample logistics, maintain meticulous records, and contribute to manuscript preparation while ensuring safety,

Qualifications

• Master's or PhD degree in Life Sciences or a related field (or equivalent experience), with proficiency in experimental design and strong hands-on laboratory skill.
• Proficient in the culture and co-culture of mammalian and immune cells, with practical expertise in maintaining cell lines and optimizing co-culture systems.

Responsibilities

• Plan, execute, troubleshoot experiments with the PI and study team.
• Establish and maintain genetically modified cancer cell models and organoids, including passaging, QC, and banking.
• Conduct cancer-immune cell co-culture and evaluate responses using flow cytometry, Luminex, ELISA, IHC, and microscopy.
• Coordinate with collaborators on sample logistics, timelines, and data sharing.
• Maintain accurate records and present updates to the PI and team.
• Manage laboratory operations (inventory, ordering, equipment maintenance) and ensure biosafety compliance.
• Prepare progress reports for lab meetings and conferences.
• Contribute to manuscript preparation.
• Oversee daily lab operations ensuring safety and compliance.

Skills

Experimental design

Cell culture

Organoid culture

Flow cytometry

Molecular techniques

Team collaboration

Education

Master's or PhD in Life Sciences or related field

Tools

PCR/qPCR

Cloning

Transfection

Western blotting

Job description

You will be responsible for planning and conducting experiments for a study exploring combination therapy in colorectal cancer. The responsibilities includes (but not limited to):

• Work closely with the PI and the study team to plan, execute, and troubleshoot experiments.

• Establishing, characterising, and maintaining genetically modified cancer cell models and human derived cancer organoid, including routine passaging, QC, and banking.

• Conduct cancer-immune cells co-culture and evaluate treatment responses using flow cytometry, Luminex, ELISA, immunohistochemistry (IHC), microscopy, and other related assays.

• Coordinate with collaborators on sample logistics, timelines, and data sharing.

• Maintain accurate, well-annotated experimental records and present regular updates to the PI and the study team.

• Manage laboratory operations (inventory, ordering, equipment maintenance) and ensure adherence to safety, biosafety, and institutional compliance requirements.

• Prepare progress reports for internal lab meetings, institutional reviews, and conferences.

• Contribute to manuscript preparation

• Manage daily laboratory operations, including inventory, ordering, and equipment maintenance, while ensuring adherence to institutional safety, biosafety, and compliance requirements.

Job Requirements

• Master or PhD degree in Life Sciences or a related field (or equivalent experience), with proficiency in experimental design and strong hands-on laboratory skill

• Proficient in the culture and co-culture of various mammalian cells and immune cells, with practical expertise in maintaining cell lines, handling primary cells and optimizing co-culture system to study cell-cell interactions and immune response in vitro

• Proficient in molecular techniques (e.g. flow cytometry, PCR/qPCR, cloning, transfection, Western blotting, etc.); experience in organoid culture is an advantage

• Responsible and organised; maintain clear records and follow SOPs and safety/biosafety practices.

• Collaborative; comfortable working in a team and learning new methods.