Postdoc: Structural Biology & NMR of Antibody PTMs

Sanofi Group; Vitry-sur-Seine; On-site; EUR 40,000 - 50,000; Full time

Job summary

Sanofi in Vitry-sur-Seine is seeking a Postdoctoral Fellow in the Biophysics & BioNMR team to study how post-translational modifications in antibody CDRs affect antigen binding and molecular stability.

The project combines experimental structural biology (2D/3D-NMR, cryo-EM, crystallography, HDX-MS) with computational modelling to illuminate PTM effects on Fab structure and recognition, supporting molecule optimization.

Qualifications

• PhD in a relevant field with strong background in NMR and structural biology.
• Hands-on protein production experience with isotopic labeling.
• Experience mapping PTMs effects on antibody fragments is a plus.

Responsibilities

• Produce and characterize samples with different levels of modification by MS, SEC, DSC and binding assays.
• Perform 2D-NMR analysis on modified and unmodified mAb/Fab samples to map chemical shift perturbations.
• Produce samples via cell-free expression.
• Structurally characterize Fab–antigen interactions by NMR, cryo-EM, crystallography and HDX-MS data.
• Design, produce and evaluate mutated Fab constructs to assess role of neighboring residues.
• Present findings to cross-functional teams and contribute to publications.

Skills

Protein production with isotope labell

NMR assignment of high MW proteins

Education

PhD in NMR spectroscopy, structural biology, biophysics or related field

Job description

Sanofi in Vitry-sur-Seine is seeking a Postdoctoral Fellow in the Biophysics & BioNMR team to study how post-translational modifications in antibody CDRs affect antigen binding and molecular stability.

The project combines experimental structural biology (2D/3D-NMR, cryo-EM, crystallography, HDX-MS) with computational modelling to illuminate PTM effects on Fab structure and recognition, supporting molecule optimization.