Postdoctoral fellow in structural biology and NMR spectroscopy

Sanofi Group; Vitry-sur-Seine; Presencial; EUR 40 000 - 50 000; Tempo integral

Resumo da oferta

Sanofi in Vitry-sur-Seine is seeking a Postdoctoral Fellow in the Biophysics & BioNMR team to study how post-translational modifications in antibody CDRs affect antigen binding and molecular stability.

The project combines experimental structural biology (2D/3D-NMR, cryo-EM, crystallography, HDX-MS) with computational modelling to illuminate PTM effects on Fab structure and recognition, supporting molecule optimization.

Qualificações

• PhD in a relevant field with strong background in NMR and structural biology.
• Hands-on protein production experience with isotopic labeling.
• Experience mapping PTMs effects on antibody fragments is a plus.

Responsabilidades

• Produce and characterize samples with different levels of modification by MS, SEC, DSC and binding assays.
• Perform 2D-NMR analysis on modified and unmodified mAb/Fab samples to map chemical shift perturbations.
• Produce samples via cell-free expression.
• Structurally characterize Fab–antigen interactions by NMR, cryo-EM, crystallography and HDX-MS data.
• Design, produce and evaluate mutated Fab constructs to assess role of neighboring residues.
• Present findings to cross-functional teams and contribute to publications.

Conhecimentos

Protein production with isotope labell

NMR assignment of high MW proteins

Formação académica

PhD in NMR spectroscopy, structural biology, biophysics or related field

Descrição da oferta de emprego

Job title: postdoctoral fellow – Structural biology / NMR spectroscopy ● Location: Vitry-sur-Seine

About the job As a Postdoctoral Fellow in the Biophysics & BioNMR / BioAnalytics team, you will investigate how Post Translational Modifications (PTMs) in antibody CDRs affects antigen binding and molecular stability. This project aims to develop a comprehensive methodology combining experimental structural biology (2D/3D-NMR, cryo-EM, crystallography, HDX-MS) with computational modelling to elucidate how PTMs in CDR modulate Fab structure and antigen recognition. Your work will directly support molecule optimization and manufacturing process development.

About Sanofi:

We’re an R&D-driven, AI-powered biopharma company committed to improving people’s lives and delivering compelling growth. Our deep understanding of the immune system – and innovative pipeline – enables us to invent medicines and vaccines that treat and protect millions of people around the world. Together, we chase the miracles of science to improve people’s lives.

Main responsibilities:

• Produce and characterize samples with different levels of modification by mass spectrometry, SEC, DSC and binding assays.
• Perform 2D-NMR analysis on modified and unmodified mAb/Fab samples to map chemical shift perturbations and identify structural signatures induced by PTMs.
• Produce samples via cell-free expression.
• Structurally characterize the Fab-antigen interaction by NMR, cryo-EM, crystallography and HDX-MS data, to provide an integrated view of how PTMs modulate binding and stability.
• Design, produce and evaluate mutated Fab constructs to determine the role of residues neighboring the PTMs binding affinity and molecular stability.
• Present findings to cross-functional teams and contribute to scientific publications.

About you

Education: PhD in NMR spectroscopy, structural biology, biophysics or a related field

Experience & technical skills:

• Hands-on expertise in protein production including U-¹³C, ¹⁵N and ²H isotope labelling and specific methyl labelling
• Proven experience in NMR assignment of high molecular weight proteins (backbone and methyl groups)

Languages:

Fluent English, intermediate level of French

Soft skills:

• Strong analytical mindset, curiosity, autonomy, and ability to collaborate within a multidisciplinary team

Why choose us?

Learn from inspiring mentors and collaborative teams while contributing to real projects that shape the future of healthcare. Launch your career with a company that invests in you — and empowers you to reimagine what’s possible. Enjoy opportunities to collaborate beyond your area of expertise, working with diverse teams across science to spark new ideas and drive breakthroughs. Work at the forefront of drug discovery, harnessing cutting‑edge AI, data, and digital platforms to push the boundaries of science. Join a workplace where diversity, equity, and inclusion are at the core, with Employee Resource Groups and leadership programs that celebrate every voice. Pursue Progress. Discover Extraordinary. Join Sanofi and step into a new era of science - where your growth can be just as transformative as the work we do. We invest in you to reach further, think faster, and do what’s never‑been‑done‑before. You’ll help push boundaries, challenge convention, and build smarter solutions that reach the communities we serve. Ready to chase the miracles of science and improve people’s lives? Let’s Pursue Progress and Discover Extraordinary – together.

At Sanofi, we provide equal opportunities to all regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity, protected veteran status or other characteristics protected by law. Pursue progress, discover extraordinary Better is out there. Better medications, better outcomes, better science. But progress doesn’t happen without people – people from different backgrounds, in different locations, doing different roles, all united by one thing: a desire to make miracles happen. So, let’s be those people.

At Sanofi, we provide equal opportunities to all regardless of race, colour, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, ability or gender identity. Watch our ALL IN video and check out our Diversity Equity and Inclusion actions at sanofi.com! We are an R&D driven, AI-powered biopharma company committed to improving people’s lives and creating compelling growth. Our team is guided by one purpose: we chase the miracles of science to improve people’s lives. We want to build a healthier, more resilient world, and turn the impossible into the possible by discovering, developing, and delivering medicines and vaccines for millions of people around the world. Discover more about us visiting www.sanofi.com or via our movie We are Sanofi Start a career that makes a difference. Reinvention is in our DNA. It’s what drove our evolution from a small French enterprise to one of the world’s leading biopharma companies. Whether it’s using AI to shorten drug‑discovery times or building trust in healthcare, you could be helping our teams make life better for patients, partners, and communities. This is where you grow your career. We open the door for you to explore new opportunities, push your limits, and connect with people who are driven by a shared purpose: we chase the miracles of science to improve people’s lives.