Postdoc: Structural Biology & NMR of Antibody PTMs

Sanofi; France; Hybrid; EUR 38.000 - 52.000; Vollzeit

Zusammenfassung

Sanofi in France invites applications for a Postdoctoral Fellow in the Biophysics & BioNMR / BioAnalytics team to study how post-translational modifications in antibody CDRs affect antigen binding and stability. The project combines experimental structural biology (2D/3D-NMR, cryo-EM, crystallography, HDX-MS) with computational modelling to map PTM effects on Fab structure and recognition.

You will produce and analyze samples with varying modification levels, perform NMR analyses on mAb/Fab

Qualifikationen

• PhD in NMR spectroscopy, structural biology, biophysics or related field.
• Hands-on protein production with isotopic labelling (U-13C, 15N, 2H) and methyl labelling.
• Experience with NMR assignment of high molecular weight proteins.

Aufgaben

• Produce and characterize samples with different levels of modification by mass spectrometry, SEC, DSC and binding assays.
• Perform 2D-NMR analysis on modified and unmodified mAb/Fab samples to map chemical shift perturbations.
• Produce samples via cell-free expression.
• Structurally characterize Fab-antigen interaction by NMR, cryo-EM, crystallography and HDX-MS data.
• Design, produce and evaluate mutated Fab constructs to determine the role of residues neighboring the PTMs.
• Present findings to cross-functional teams and contribute to scientific publications.

Kenntnisse

Isotopic labelling

NMR spectroscopy

Analytical mindset

Collaboration

Ausbildung

PhD in NMR spectroscopy or related field

Tools

Mass spectrometry

SEC

DSC

NMR analysis

Cryo-EM

Crystallography

HDX-MS

Cell-free expression

Jobbeschreibung

Sanofi in France invites applications for a Postdoctoral Fellow in the Biophysics & BioNMR / BioAnalytics team to study how post-translational modifications in antibody CDRs affect antigen binding and stability. The project combines experimental structural biology (2D/3D-NMR, cryo-EM, crystallography, HDX-MS) with computational modelling to map PTM effects on Fab structure and recognition.

You will produce and analyze samples with varying modification levels, perform NMR analyses on mAb/Fab