Breast Cancer Research Engineer: Organoids, CRISPR, Imaging

EURAXESS Ireland; Marseille; Hybrid; EUR 40,000 - 54,000; Full time

Job summary

The INSERM U1068 Marseille Cancer Research Centre is seeking a highly qualified Biologist to investigate early mechanisms of breast tumorigenesis. The project uses lineage-tracing in luminal epithelial cells, organoid cultures, and mouse xenograft models to understand early tumor transformation.

The successful candidate will work in the Epithelial Stem Cells and Cancer team, perform CRISPR-based screens, and employ confocal microscopy and flow cytometry to analyze results, contributing to

Qualifications

• PhD in Biological sciences or related field.
• Experience in organoid culture and maintenance.
• Experience with orthotopic grafts in mammary gland models.
• Familiarity with flow cytometry and histology analyses.
• Strong microscopy experience, esp. confocal.
• Experience with CRISPR-based functional screens.
• Ability to work in English and in a multidisciplinary team.

Responsibilities

• Investigate early events of breast tumor initiation and progression.
• Utilize organoid cultures and mouse xenograft models.
• Perform CRISPR-based functional screens to identify drivers.
• Conduct confocal microscopy and spectral flow cytometry analyses.
• Characterize models within the Epithelial Stem Cells and Cancer team.

Skills

Organoid culture maintenance

Orthotopic graft implantation

Transplanted animals monitoring

Tissue collection & processing

Confocal microscopy imaging

CRISPR-based functional screens

English communication

Independent work in multidisciplinary팀

Technical autonomy & organization

Scientific initiative

Education

PhD or equivalent

Tools

Flow cytometry instruments

CRISPR screening platforms

Job description

The INSERM U1068 Marseille Cancer Research Centre is seeking a highly qualified Biologist to investigate early mechanisms of breast tumorigenesis. The project uses lineage-tracing in luminal epithelial cells, organoid cultures, and mouse xenograft models to understand early tumor transformation.

The successful candidate will work in the Epithelial Stem Cells and Cancer team, perform CRISPR-based screens, and employ confocal microscopy and flow cytometry to analyze results, contributing to