Research Engineer - Preclinical Sarcoma Models & Organoids

Institut Curie; Paris; Hybrid; EUR 42,000 - 66,000; Full time

Job summary

Institut Curie’s RACER team in Paris advances preclinical modeling for rare cancers, focusing on sarcomas and cancers of unknown primary using cell lines, organoids, and PDX. You will work under Dr. Sarah Watson and collaborate with core facilities at Institut Curie.

The role covers establishing models from patient material, developing 3D culture systems, and performing molecular analyses to support target validation and drug screening in a biosafety 2 environment.

Responsibilities

• Establish and characterize sarcoma-derived cell lines, organoids, and PDX models from patient samples
• Develop and optimize 3D culture systems and co-cultures with stromal or immune cells
• Maintain, monitor, and annotate models and associated biobanks
• Perform functional assays, including viability, dose–response, and drug screening studies
• Extract nucleic acids and proteins and perform quality assessment
• Prepare sequencing samples and liaise with sequencing platforms
• Conduct RT-qPCR, Western blotting, flow cytometry, immunofluorescence, and immunohistochemistry
• Perform genetic engineering using transfection, lentiviral transduction, and CRISPR/Cas9
• Contribute to quality control and preliminary data analysis
• Ensure experimental traceability, data management, and regulatory compliance
• Participate in inventory management, purchasing, and equipment maintenance
• Train and support students and new team members in cell culture and preclinical modeling techniques
• Present research findings and contribute to publications, grant reports, and regulatory documentation

Job description

Institut Curie’s RACER team in Paris advances preclinical modeling for rare cancers, focusing on sarcomas and cancers of unknown primary using cell lines, organoids, and PDX. You will work under Dr. Sarah Watson and collaborate with core facilities at Institut Curie.

The role covers establishing models from patient material, developing 3D culture systems, and performing molecular analyses to support target validation and drug screening in a biosafety 2 environment.