Senior Scientist, Bioanalytical Immunology & LBAs

Flagship Pioneering, Inc.; Cambridge (MA); On-site; USD 126,000 - 182,000; Full time

Benefits offered by this job

Healthcare coverage

Annual incentive program

Retirement benefits

Job summary

Serif Biomedicines seeks a Senior Scientist, Bioanalytical Sciences (Innate Immunity, LBA & Flow Cytometry) to lead hands-on bioanalytical assay development for nucleic acid therapies, including LNPs, with a focus on innate immune responses. You will mentor junior scientists and collaborate across CMC, molecular biology, and cell biology teams.

Requires a Ph.D. with 5+ years; expertise in innate immunology, multicolor flow cytometry, ddPCR, LBAs; experience with LNPs and biodistribution; strong

Qualifications

• Ph.D. in immunology, biochemistry, pharmaceutical sciences, cell biology or related field with 5+ years of industry experience.
• Demonstrated expertise in innate immune signaling and nucleic acid sensing pathways for DNA and mRNA.
• Proficiency in multicolor flow cytometry, ddPCR, ligand binding assays, leukopak processing and isolation of PBMCs.

Responsibilities

• Design, develop and execute cell-based immunoassays, flow cytometry based assays and LBAs to assess immunogenicity to LNP delivered nucleic acid cargoes in vitro and in vivo.
• Apply knowledge of innate immunology and establish in vitro screening assays for various nucleic acid modalities and establish criteria for release of cargo for pre-clinical research.
• Collaborate closely with CMC, molecular biology and cell biology teams to screen nucleic acid cargoes and ensure timely delivery of high-quality data and summarize results.
• Ensure development, qualification, and validation of bioanalytical assays in compliance with applicable guidelines.
• Design, optimize, and execute biodistribution studies (flow cytometry and histological techniques) for LNP/nucleic acid therapies.
• Design, establish and execute assays for assessing various in vivo immunogenicity predictors for LNP/nucleic acid therapies including biomarkers, cytokines, complement activation and anti-drug antibodies in rodents and NHPs.
• Design, develop, optimize, and execute bioanalytical assays to support nonclinical studies, including PK, PD, biomarker, and immunogenicity endpoints.
• Execute test procedures such as sample extraction and measurement by Flow Cytometry, ELISA, MSD, Luminex, bDNA, ddPCR and perform data interpretation.
• Analyze and summarize scientific data and maintain timely documentation and completion of electronic lab notebooks in compliance with industry guidelines.
• Present at project team meetings and to relevant stakeholders.
• Mentor, coach, and manage junior scientists.

Skills

Innate immunology

Flow cytometry

ddPCR

Ligand binding assays

Nucleic acid therapeutics

Mentoring

Education

Ph.D. in immunology/biochemistry/related field

Tools

Multicolor flow cytometer

ddPCR system

ELISA/MSD/Luminex

Job description

Serif Biomedicines seeks a Senior Scientist, Bioanalytical Sciences (Innate Immunity, LBA & Flow Cytometry) to lead hands-on bioanalytical assay development for nucleic acid therapies, including LNPs, with a focus on innate immune responses. You will mentor junior scientists and collaborate across CMC, molecular biology, and cell biology teams.

Requires a Ph.D. with 5+ years; expertise in innate immunology, multicolor flow cytometry, ddPCR, LBAs; experience with LNPs and biodistribution; strong