Senior Protein Design Associate — Express & Characterize Antigens

Moderna; Cambridge (MA); Presencial; USD 74 000 - 118 000; Tempo integral

Vantagens oferecidas por esta oferta de emprego

Competitive healthcare and voluntary福利

Well‑being resources including fitness

Family planning benefits (fertility, -

Sabbatical and global recharge days

Generous paid time off

Resumo da oferta

Moderna in Cambridge, MA is seeking a Senior Research Associate for the Protein Design team to advance vaccine and therapeutic programs through protein production, purification, and extensive characterization. The role demands expertise in mammalian and bacterial expression, purification, and analytical biochemistry/biophysics, with collaboration across multidisciplinary teams.

The position is onsite, with opportunities to contribute to AI-assisted workflows and high-throughput analyses, driving

Qualificações

• Bachelor's degree with 2+ years of relevant experience.
• Hands-on experience with recombinant protein expression in both mammalian and bacterial (E. coli) systems.
• Preferred: 4+ years of relevant experience, or a Master's with 2+ years of relevant experience.

Responsabilidades

• Execute and optimize recombinant protein expression workflows in mammalian and bacterial (E. coli) expression systems.
• Perform transient mammalian cell culture and transfection for protein production.
• Conduct recombinant protein purification using chromatography-based methods including affinity, ion exchange, and size‑exclusion chromatography utilizing FPLC systems.
• Support development and optimization of expression, purification, and formulation conditions for challenging proteins and complex antigens.
• Characterize proteins using biochemical and biophysical techniques including SDS-PAGE, western blotting, SEC-MALS, DSC, DSF, BLI, Mass Photometry, and flow cytometry‑based approaches.
• Evaluate protein purity, stability, oligomeric state, aggregation, and biomolecular interactions to support candidate assessment and advancement.
• Analyze, interpret, and summarize experimental data and communicate findings through presentations, technical reports, and project team discussions.
• Develop and implement AI‑assisted workflows for high‑throughput protein screening and characterization.
• Leverage AI to accelerate analysis of protein screening datasets and identify high‑performing candidates.
• Collaborate closely with scientists and cross‑functional teams to support project goals, timelines, and deliverables.
• Identify technical challenges and troubleshoot protein expression, purification, and characterization workflows.
• Support implementation and evaluation of new protein production and characterization technologies to improve workflow efficiency.
• Maintain accurate experimental records and support inventory management, reagent tracking, and logistics coordination for partnered programs.

Conhecimentos

Protein expression

Mammalian expression

Bacterial expression (E. coli)

Protein purification

Biochemical characterization

Biophysical characterization

Data analysis

Communication skills

Formação académica

Bachelor's degree in Biochemistry, Molecular Biology, Biomedical Science, Protein Engineering, Chemical Biology, or related discipline

Ferramentas

FPLC systems

SDS-PAGE

Western blot

SEC-MALS

DSC

DSF

BLI

Mass Photometry

Descrição da oferta de emprego

Moderna in Cambridge, MA is seeking a Senior Research Associate for the Protein Design team to advance vaccine and therapeutic programs through protein production, purification, and extensive characterization. The role demands expertise in mammalian and bacterial expression, purification, and analytical biochemistry/biophysics, with collaboration across multidisciplinary teams.

The position is onsite, with opportunities to contribute to AI-assisted workflows and high-throughput analyses, driving