Senior Associate Scientist - CAR T mRNA Engineering

PharmaEssentia Innovation Research Center; Bedford (MA); On-site; USD 110,000 - 150,000; Full time

Job summary

PharmaEssentia Corporation is seeking a Senior Associate Scientist to join the Cell Therapy team and contribute to the development of novel immunotherapies for immunology and oncology. The role centers on mRNA-based CAR T cell engineering and immunoassay characterization.

You will generate data to support key project decisions, interpret results, and design experiments. Working at PharmaEssentia Innovation Research Center (PIRC), you will collaborate across multidisciplinary teams in a

Qualifications

• BS/MS in immunology, biology, oncology or related discipline with 6+ years (BS) or 4+ years (MS) of industry experience.
• Hands-on experience with primary human immune cells and cancer cell lines.
• T cell transfection via mRNA electroporation.
• Immunophenotyping using flow cytometry (multicolor).
• Cytotoxicity assays, multiplex ELISA, live-cell analysis.
• Proficiency with FlowJo, GraphPad Prism, and related software.

Responsibilities

• Perform primary immune cell isolation and culture, and cancer cell line culture.
• Conduct mRNA-based CAR T cell engineering using electroporation and tLNP.
• Lead functional characterization of CAR T cells with immunoassays.
• Analyze data and document work in ELN system.
• Present results at team meetings and external conferences.
• Collaborate with cross-functional project teams.
• Maintain a clean, safe BSL2/BSL2+ lab environment.

Skills

Primary immune cell culture

T cell transfection

Flow cytometry

ELISA

Live-cell imaging

Data analysis (FlowJo)

Data analysis (GraphPad Prism)

Immunophenotyping

Multicolor flow cytometry

Education

Bachelor's or Master's in immunology/biology/oncology

Tools

BD Fortessa flow cytometer

Lonza 4D-Nucleofector

Meso Scale Discovery plate reader

xCELLigence RTCA

Job description

PharmaEssentia Corporation is seeking a Senior Associate Scientist to join the Cell Therapy team and contribute to the development of novel immunotherapies for immunology and oncology. The role centers on mRNA-based CAR T cell engineering and immunoassay characterization.

You will generate data to support key project decisions, interpret results, and design experiments. Working at PharmaEssentia Innovation Research Center (PIRC), you will collaborate across multidisciplinary teams in a