Senior Associate Scientist, CAR T Cell Therapy

PharmaEssentia U.S.A.; Bedford (MA); On-site; USD 110,000 - 140,000; Full time

Job summary

PharmaEssentia Corporation is seeking a Senior Associate Scientist to join the Cell Therapy team and contribute to the development of novel immunotherapies. The role focuses on mRNA-based CAR T cell engineering and immunoassay characterization to support discovery projects and technology evaluations.

The candidate will generate data to aid key decisions, interpret results, and design experiments in a dynamic, fast-paced environment. Strong bench experience and collaboration are essential.

Qualifications

• Senior Associate Scientist: B.S./M.S. in immunology, biology, oncology or related discipline with BS 6+ years or MS 4+ years of relevant industry experience
• Hands-on experience with primary human immune cell and cancer cell line culture, T cell transfection via mRNA electroporation
• Immunophenotyping using flow cytometry, cytotoxicity assays, multiplex ELISA, live-cell analysis

Responsibilities

• Perform primary immune cell isolation and culture, and cancer cell line culture
• Perform mRNA-based CAR T cell engineering using electroporation and tLNP
• Lead functional characterization of CAR T cells with immunoassays
• Data analysis and thorough documentation of work in ELN
• Present results at team meetings and external scientific conferences
• Work cross-functionally within multi-disciplinary project teams
• Maintain a safe, clean laboratory in a BSL2/BSL2+ setting

Skills

Primary human immune cell culture

T cell transfection via mRNA electropo

Multicolor flow cytometry

Cytotoxicity assays

Multiplex ELISA

Live-cell analysis (Incucyte)

Data analysis (FlowJo, GraphPad)

Education

B.S./M.S. in immunology/biology/oncology or related

Tools

BD Fortessa flow cytometer

Lonza 4D-Nucleofector

Meso Scale Discovery plate reader

xCELLigence RTCA

Job description

PharmaEssentia Corporation is seeking a Senior Associate Scientist to join the Cell Therapy team and contribute to the development of novel immunotherapies. The role focuses on mRNA-based CAR T cell engineering and immunoassay characterization to support discovery projects and technology evaluations.

The candidate will generate data to aid key decisions, interpret results, and design experiments in a dynamic, fast-paced environment. Strong bench experience and collaboration are essential.