Scientist, Lentiviral Vector Cell Line Development

Asimov, Inc.; Boston, Northern (MA, KY); Hybrid; USD 100,000 - 140,000; Full time

Job summary

Asimov, Inc. in Boston, MA is seeking a Scientist for the Lentiviral Vector Cell Line Development team.

You will engineer stable producer cell lines for LV therapeutics, optimize upstream processes, and contribute to scale-up using Ambr15 and Ambr250 systems. Responsibilities include transfection, clone generation, characterization, cell banking, and assays for LV quantity, potency, and infectivity; collaborate with Synthetic Biology and Process Development; design experiments, analyze data, and

Qualifications

• PhD or equivalent with hands-on LV production experience in industry.
• Extensive viral transduction experience including T-cell transduction and gene expression analyses.
• Experience with Ambr15/Ambr250 or benchtop viral vector bioreactor processes.
• Downstream purification or genome engineering related to virus production is a plus.
• Strong written and verbal communication skills.

Responsibilities

• Perform LV stable producer cell-line development: transfection, pool generation, cloning, characterization, and cell banking.
• Assess LV preparations for quantity, potency, and infectivity.
• Support novel workflows and cross-functional collaboration across LV CLD, Synthetic Biology, and Process Development teams.
• Contribute to process development, scale-up, and LV analytical assay development across Ambr systems.
• Design experiments, analyze data, troubleshoot, and maintain detailed records.
• Represent LV CLD on project teams and communicate results and plans.

Skills

Lentiviral vector production

Cell line development

Transfection

Flow cytometry

ddPCR/qPCR

Education

PhD in Biological Engineering

MS in related field

Bachelor's in related field

Tools

Ambr15

Ambr250

Job description

Our mission at Asimov is to advance humanity's ability to design living systems, enabling biotechnologies with outsized benefit to society. We're developing a mammalian synthetic biology platform––from cells to software––to enable the design and manufacture of next-generation therapeutics.

We are seeking a Scientist to join our Lentiviral Vector Cell Line Development team in Boston, MA. The ideal candidate has a proven track record developing lentiviral vector (LV) packaging and stable producer cell lines in an industry setting. In this role, you will play a central part in engineering stable producer cell lines for LV therapeutics while driving upstream process optimization and scale-up. This is a unique opportunity to work at a nimble, forward-thinking synthetic biology startup and help build the future of biological engineering.

About the Role:

• Perform Lentiviral vector stable producer cell line development processes including transfection, pool generation, cloning, characterization, and cell banking using state-of-the-art equipment.

• Perform assays to assess quantity, potency and infectivity of lentiviral vector preparations.

• Support innovation through development of novel workflows and applications.

• Support research projects to develop viral vector therapeutics with enhanced quality attributes such as infectivity, potency and/or cell targeting.

• Work effectively as part of a cross-functional team in support of the LV CLD platform and in collaboration with Synthetic Biology and Process Development teams.

• Contribute to process development efforts, including scale-up and characterization across Ambr15, Ambr250, and benchtop systems, as well as LV analytical assay development.

• Design experiments, analyze data, troubleshoot, and maintain detailed records.

• Represent LV CLD on project teams, making decisions for cell line development activities and effectively communicating results, progress and plans.

About You:

• You have a PhD in Biological Engineering, Chemical Engineering, Cell Biology, Molecular Biology, or a related field with 0-2 years of hands-on industry experience with Lentiviral vector production and cell line development in support of gene therapies; alternatively, a MS with 3-4 years or Bachelors with 6-8 years of this type of experience.

• Extensive experience performing viral transductions, including T-cell transduction assay, flow cytometry, and quantitative gene expression analyses (ddPCR or qPCR).

• Experience with Ambr15, Ambr250, and/or benchtop scale viral vector bioreactor processes.

• Experience with downstream purification of Lentiviral vectors, viral genome engineering, and/or host cell genome engineering related to virus production or producer cell line technologies is highly desired.

• Strong written and verbal communication skills required.

We're fueled by a vision to transform biological engineering into a fully-fledged engineering discipline. Should you join our team, you will grow with a constantly evolving organization and push the frontiers of synthetic biology. Company culture is key to Asimov, and ours is a culture of recombination; we believe that our mission can only be achieved by bringing together a diverse team with a mixture of backgrounds and perspectives.