Scientist II, CRISPR-X - Gene Insertion

Crispr Therapeutics Inc in; Boston (MA); On-site; USD 130,000 - 140,000; Full time

Benefits offered by this job

Equity

Bonus eligible

On-site in Boston

Job summary

CRISPR Therapeutics, Inc. in Boston, MA, is seeking a Scientist II to advance our CRISPR-X program and next-generation gene editing therapies. You will lead experiments, design constructs for CAR T and other immunotherapies, and work with molecular biology techniques.

You will collaborate across teams, interpret data, and contribute to translational assays evaluating gene editing in vitro and in vivo. This on-site role offers a competitive base salary, bonus eligibility, and equity in a

Qualifications

• PhD in biology, bioengineering, immunology, or related discipline.
• 2–5+ years of professional experience (or 10–12+ years without PhD).
• Strong problem-solving and independent work ethic.

Responsibilities

• Lead design and optimization of next-generation gene editing strategies using CRISPR-based tools and beyond.
• Design, execute, and interpret molecular biology experiments including cloning, qPCR/ddPCR, NGS, RNA and DNA analyses, and cell culture.
• Apply immune biology to guide CAR T construct design, target selection, and phenotype interpretation in vivo.
• Review literature to develop novel gene editing and immunology solutions.
• Develop translational assays to evaluate gene editing and CAR approaches across in vitro and in vivo systems.

Skills

CAR T engineering

Gene editing

Molecular biology

Cell culture

Data interpretation

Education

PhD in biology or related field

Tools

qPCR

NGS

Cloning

Job description

Job Description:

Founded over a decade ago,CRISPR Therapeuticsis a leading biopharmaceutical company focused on developing transformative gene-based medicines for serious human diseases. The Company has evolved from a pioneering research-stage organization into an industry leader, marking a historic milestone with the approval of CASGEVY®(exagamglogene autotemcel [exa-cel]), the world's first CRISPR-based therapy, approved for eligible patients with sickle cell disease and transfusion-dependent beta thalassemia.CRISPR Therapeuticsis advancing a broad and diversified pipeline across hemoglobinopathies, cardiovascular, autoimmune, oncology, regenerative medicine and rare diseases. The Company continues to expand its leadership in gene editing through the development of SyNTase™ editing, a novel and proprietary gene-editing platform designed to enable precise, efficient, and scalable gene correction. To accelerate and expand its impact,CRISPR Therapeuticshas established strategic collaborations with leading biopharmaceutical partners, includingVertex Pharmaceuticals.CRISPR Therapeutics AGis headquartered inZug,Switzerland, with its wholly-ownedU.S.subsidiary,CRISPR Therapeutics, Inc., and R&D operations based inBoston, MassachusettsandSan Francisco,California.

Position Summary

Are you passionate about developing next-gen CRISPR therapies? We are seeking a highly motivated and creative Scientist II to grow our diverse, innovation-focused CRISPR-X team that pioneers novel gene editing therapies for human disease. We are part of CRISPR Therapeutics, a leading gene editing company with a broad clinical portfolio. The successful candidate will be an integral part of our team, whose aim is to research, develop and advance the next generation of genome editing modalities and genetic therapies for a wide range of human indications. The position requires enthusiasm, passion, outstanding skills in the art, attention to detail, and a desire to create new medicines for patients

Responsibilities

• Lead, design, and optimize research with the aims of exploring and developing next-generation gene editing strategies, primarily gene insertions, using CRISPR-based tools and beyond, and synthetic immunology.
• Design, execute, and interpret research requiring molecular biology techniques including cloning, qPCR/ddPCR, NGS, RNA and DNA analysis, cell culture, and other cell-based assays.
• Use knowledge of immune cell biology to guide construct design, target selection, and interpretation of phenotypic outcomes of in-vivo CAR T and other immune studies.
• Dissect published literature to find novel solutions to gene editing and immunology problems.
• Develop a deep understanding of the biology underlying new disease indications and design translational assays and experimental strategies to enable evaluation of gene editing and CAR approaches across in vitro and in vivo systems.

Minimum Qualifications

• Prior experience with gene editing, including experience applying and developing next generation gene editing technologies, such as recombinases, retrotransposons or other integration approaches, in an academic and/or industry setting, with a track record demonstrating significant contributions.
• Hands-on experience with chimeric antigen receptor (CAR) T cell engineering, including CAR construct design, gene editing and/or transgene delivery in primary human T cells, and functional characterization of the resulting CAR-T cells.
• Ph.D. in biology, bioengineering, immunology, or related discipline with 2-5+ years of professional experience, or non-PhD and 10-12+ years of progressive, relevant experience.
• Outstanding problem-solving skills and attitude to think outside the box.
• Excellent writing, reviewing, and presentation skills.
• Challenges oneself continuously to enhance scientific capabilities.
• Ability to work independently as well as collaboratively in a results-oriented research team environment.
• Must possess critical thinking and self-reliance to solve tasks independently.
• Self-motivated and scientifically rigorous, with outstanding organization and multitasking capability.
• Entrepreneurial spirit showing resourcefulness, accountability, agility to pivot, transparency, and productivity.

Preferred Qualifications

• Prior experience with protein and/or RNA engineering.
• Prior experience with diverse delivery technologies, including transfection/nucleofection, lipid nanoparticles, and viral vectors/particles.
• Prior experience designing and executing in vivo studies, including downstream tissue processing.
• Experience managing and mentoring junior scientists.

Results Orientation – Delivering progress toward our mission. Sense of urgency in solving problems.

Due to the nature of their work, our manufacturing and lab-based positions are located fully on-site.

Scientist II: Base pay range of $130,000 to $140,000+ bonus, equity and benefits

The range provided is CRISPR Therapeutics' reasonable estimate of the base compensation for this role. The actual amount will be based on job-related and non-discriminatory factors such as experience, training, skills, and abilities.

CRISPR Therapeutics, Inc. is committed to equal employment opportunity and non-discrimination for all employees and qualified applicants without regard to a person's race, color, gender, age, religion, national origin, ancestry, disability, veteran status, genetic information, sexual orientation or any characteristic protected under applicable law.