Scientist II, Conjugation

Soufflé Therapeutics; Watertown (MA); On-site; USD 140,000 - 210,000; Full time

Job summary

Soufflé Therapeutics, a Watertown, MA biopharmaceutical company, seeks a Scientist II in Protein Science – Conjugation. You will contribute hands-on bioconjugation expertise to advance conjugation methods and molecular modalities across internal programs and collaborations. This role offers ownership from day one in a growing biotech setting.

You will lead conjugation work, develop new payload modalities, and perform rigorous analytical characterizations to support in vivo and in vitro studies.

Qualifications

• 6+ years of relevant experience (academic and industry combined).
• Hands-on experience with protein bioconjugation, particularly antibody-drug conjugates.
• Experience purifying proteins using affinity chromatography, ion exchange chromatography, and tangential flow filtration.
• Proficiency with analytical techniques: SEC-HPLC, IEX-HPLC, RP-HPLC, SDS-PAGE, MS.

Responsibilities

• Prepare novel bioconjugates for in vitro and in vivo studies.
• Lead conjugation work for internal programs and collaborations.
• Advance conjugation chemistry research into new modalities and payloads.
• Characterize conjugates using SEC-HPLC, SDS-PAGE, and mass spectrometry.
• Represent the conjugation team in cross-functional meetings.

Skills

Protein bioconjugation

Antibody-drug conjugates

Protein purification

Analytical chemistry

Mass spectrometry

Education

Ph.D. in Chemistry

BS with 10+ years' conjugation experience

Tools

SEC-HPLC

IEX-HPLC

RP-HPLC

SDS-PAGE

Mass spectrometry

Job description

Soufflé Therapeutics is a biotech company focusing on the cell-type specific delivery of genetic medicines. It's team of driven scientists is making a mark in delivery of oligonucleotides to extrahepatic tissues. We thrive in a purpose-building lab and office space in Watertown. Come and join us in our quest!

Position Summary:

Soufflé Therapeutics is growing its conjugation capabilities to enable state of the art conjugation methodologies to new ligand and payload modalities, expanding internal programs, and new collaborations. As a Scientist II in Protein Science – Conjugation, you'll add hands-on bioconjugation expertise to advance new conjugation methods and enable new molecular modalities across internal programs and external collaborations. If you want to help shape how a growing biotech builds its conjugation chemistry and purification capabilities, this role gives you real ownership from day one.

Key Responsibilities

• Leverage knowledge of protein and conjugation chemistry to prepare novel bioconjugates for in vitro and in vivo studies.
• Lead conjugation work supporting internal programs and collaborations, from planning through execution.
• Drive conjugation chemistry research into new conjugation modalities, payloads, and conjugate formats.
• Perform characterization of conjugates using techniques such as SEC-HPLC, SDS-PAGE, and mass spectrometry.
• Represent the conjugation team in prioritization and cross-functional collaboration meetings.

What Success Looks Like

• In 3 months: You've mastered our core conjugation workflows and techniques and understand the background of current projects
• In 6 months: You're independently executing conjugation experiments, and rigorously analyzing and interpreting data
• In 12 months: Your experimental work is making a measurable impact on company objectives – improving our conjugation process or enabling new payloads or workflows – and you know how to get things done across the organization

Qualifications — Required

• 6+ years of relevant experience (academic and industry combined)
• Hands-on experience with protein bioconjugation, particularly antibody-drug conjugates
• Hands-on experience with protein purification methods, such as affinity chromatography, ion exchange chromatography, and tangential flow filtration
• Proficiency in analytical techniques for characterizing proteins and protein conjugates, such as SEC-HPLC, IEX-HPLC, RP-HPLC, SDS-PAGE, and mass spectrometry

Qualifications — Preferred

• Experience optimizing chemical and enzymatic bioconjugation methods, including site-specific conjugation
• Experience with multi-gram-scale bioconjugation and/or purification, and with HPLC method development, maintenance, and troubleshooting

Education

• Ph.D. in Chemistry, Biochemistry, Chemical Biology, Bioengineering or MS with 6-10 years of industry experience with conjugation OR a BS with 10+ years' experience

At Soufflé Therapeutics, we are intentional about building an equitable culture. How we treat our people is reflective of this focus. This is an onsite position in Watertown, MA (US) with flexibility as needed for collaboration and external engagements.