Scientist - II

Net2Source Inc.; South San Francisco (CA); On-site; Full time

Job summary

A leading company is seeking a motivated Scientist to join their Protein Science department. The role involves supporting the production of recombinant proteins and involves advanced techniques in cell culture and protein expression. Ideal candidates will have a strong educational background and relevant industry experience.

Qualifications

• B.S. or M.S. in Biochemistry or related field required.
• 3 years (B.S.) or 1 year (M.S.) experience in relevant field.
• Comfortable with cell culture work and sterile techniques.

Responsibilities

• Support protein production and maintain CHO cell cultures.
• Carry out transient and stable transfections.
• Conduct analytical assays using HPLC and other methods.

Skills

Mammalian cell culture techniques

Protein expression

Analytical measurements

Aseptic techniques

Communication skills

Education

B.S. degree in Biochemistry, Cell/Molecular Biology, or related field

M.S. degree in similar fields

Tools

HPLC

SDS-PAGE

Job description

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This range is provided by Net2Source Inc.. Your actual pay will be based on your skills and experience — talk with your recruiter to learn more.

Base pay range

$50.00/hr - $53.00/hr

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Duration: 12+ Months contract (Extendable)

Education and experience required:

• B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields

Must haves:

• Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.

• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).

• Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.

Nice to haves/preferred:

• Experience with high-throughput systems and automation.

• Experience with batch purification using a variety of scaffolds and basic QC.

• Industry experience is preferred.

Software skills:

• Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.

• Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.

• Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.

• Strong communication, organizational, and interpersonal skills.

• Able to maintain a “can-do” attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs.

• Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials

• Able to troubleshoot cell culture system and think critically about experiments.

• Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work.

Responsibilities:

We are looking for a motivated scientist to be an integral part of the Protein Science department within Discovery Biologics at client Research Labs (MRL)- South San Francisco.

• The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and novel protein modalities to enable all aspects of Early Discovery.

• Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.

• Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.

Job responsibilities in detail:

• Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.

• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.

• Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.

• Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.

• Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.

• Maintain and troubleshoot mammalian cell culture systems.

• Document protein production processes, prepare reports and communicate results

Seniority level

• Seniority level

  Mid-Senior level

Employment type

• Employment type

  Contract

Job function

• Job function

  Manufacturing, Science, and Research

• Industries

  Pharmaceutical Manufacturing, Medical Equipment Manufacturing, and Biotechnology Research

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