Associate Scientist - II #Protein Science

Net2Source Inc.; South San Francisco (CA); On-site; Full time

Job summary

A leading pharmaceutical client seeks a Scientist II (Associate) with expertise in protein expression and purification for a 12+ months contractual opportunity in South San Francisco. Ideal candidates will have a background in biochemistry or related fields and experience with mammalian cell culture and purification techniques. This position offers a competitive salary and the chance to contribute significantly to early discovery projects in a dynamic environment.

Qualifications

• B.S. or M.S. degree with required years of experience in biochemistry or related field.
• Experience with mammalian cell culture techniques and protein expression.
• Ability to multi-task and manage concurrent projects.

Responsibilities

• Maintain and propagate CHO cells to support protein production projects.
• Perform transient and stable transfections for protein expression.
• Document processes and communicate results effectively.

Skills

Mammalian cell culture techniques

Protein expression

Purification techniques

Attention to detail

Education

B.S. degree in Biochemistry, Cell/Molecular Biology, or related field

M.S. degree in related field

Tools

HPLC

SDS-PAGE

UPLC

Job description

Get AI-powered advice on this job and more exclusive features.

This range is provided by Net2Source Inc.. Your actual pay will be based on your skills and experience — talk with your recruiter to learn more.

Base pay range

$45.00/hr - $52.00/hr

Direct message the job poster from Net2Source Inc.

Sr. Pharma Recruiter @ Net2Source Inc. | Master Degree in Biotechnology, Technical Recruiting, Biology subject expert

Hello

Net2Source is currently seeking a Scientist II (Associate) with expertise in protein expression and purification for a contractual opportunity with a leading pharmaceutical client. If you're interested, we encourage you to apply!

Please find below the job description.

Job title: Scientist - II (Associate)

Duration: 12+ months.

Description

We are looking for a motivated scientist to be an integral part of the Protein Science department within Discovery Biologics at *** at South San Francisco.

• The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and Client protein modalities to enable all aspects of Early Discovery.

• Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.

• Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.

Job responsibilities in detail:

• Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.

• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.

• Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.

• Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.

• Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.

• Maintain and troubleshoot mammalian cell culture systems.

• Document protein production processes, prepare reports and communicate results

Qualifications

Education and experience required:

• B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields

Must haves:

• Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.

• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).

• Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.

Nice to haves/preferred:

• Experience with high-throughput systems and automation.

• Experience with batch purification using a variety of scaffolds and basic QC.

• Industry experience is preferred.

Software skills:

• Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.

• Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.

Seniority level

• Seniority level

  Associate

Employment type

• Employment type

  Contract

Job function

• Job function

  Research and Analyst

• Industries

  Pharmaceutical Manufacturing and Biotechnology Research

Referrals increase your chances of interviewing at Net2Source Inc. by 2x

Sign in to set job alerts for “Scientist” roles.

Foster City, CA $135,000.00-$185,000.00 2 weeks ago

Scientist I/II, Analytical Development (Contract to Hire)

RESEARCH SCIENTIST SUPERVISOR I (CHEMICAL SCIENCES)

Alameda, CA $9,254.00-$11,868.00 1 week ago

Foster City, CA $146,540.00-$189,640.00 2 weeks ago

Staff Scientist - Dual Scope (Environmental / Property Condition Assessment)

Scientist 1/2/Senior Scientist, Analytical Development

Menlo Park, CA $100,000.00-$150,000.00 3 weeks ago

Hayward, CA $125,000.00-$150,000.00 3 days ago

San Leandro, CA $125,000.00-$150,000.00 3 days ago

South San Francisco, CA $170,000.00-$200,000.00 2 weeks ago

South San Francisco, CA $140,000.00-$150,000.00 2 weeks ago

Assistant/Associate/Full Researcher Positions Available

South San Francisco, CA $150,000.00-$165,000.00 4 days ago

San Francisco, CA $136,000.00-$212,800.00 2 weeks ago

Palo Alto, CA $60,000.00-$85,000.00 3 weeks ago

Senior Scientist I - CMC BioProcess Purification Development

South San Francisco, CA $94,000.00-$178,500.00 4 days ago

We’re unlocking community knowledge in a new way. Experts add insights directly into each article, started with the help of AI.