Scientist

Net2Source Inc.; South San Francisco (CA); On-site; Full time

Job summary

A leading biopharmaceutical company seeks a 'Scientist - II' to engage in the production of recombinant proteins and monoclonal antibodies. The role involves maintaining mammalian cells, performing complex transfections, and ensuring high-quality data through meticulous documentation. Ideal candidates will possess relevant degrees with industry experience and strong communication skills.

Qualifications

• B.S. degree in relevant field plus 3 years of experience, or M.S. degree with 1 year of experience.
• Prior experience in mammalian cell culture and transfection techniques is essential.
• Strong communication and organizational skills required.

Responsibilities

• Maintain and propagate CHO cells in suspension culture independently.
• Perform transient and stable transfections for protein expression.
• Execute fed-batch experiments and analytical measurements for cell growth.

Skills

Mammalian cell culture techniques

Protein expression in mammalian cells

Purification techniques

Analytical measurement techniques

Aseptic techniques

Communication skills

Education

B.S. degree in Biochemistry, Cell/Molecular Biology, or related fields

M.S. degree in similar fields (preferred)

Tools

Biological data collection tools (Vicell, metabolite analyzers)

Data management software (Dotmatics, Benchling)

Microsoft Office Suite (Word, Excel, PowerPoint)

Job description

Net2Source Inc. is an award-winning total workforce solutions company recognized by Staffing Industry Analysts for our accelerated growth of 300% in the last 3 years with over 5500+ employees globally, with over 30+ locations in the US and global operations in 32 countries. We believe in providing staffing solutions to address the current talent gap – Right Talent – Right Time – Right Place – Right Price and acting as a Career Coach to our consultants.

Company: One of Our Clients

Job Description:

Title: Scientist - II

Location: South San Francisco, CA

Duration: 12+ Months (Extendable)

Pay Rate: $50.00 - 53.45/hr. on W2

Qualifications:

Education and experience required:

• B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields

Must haves:

• Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.

• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).

• Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.

Key skills:

• Maintain and propagate CHO cells in suspension culture independently

• Perform transient and stable transfections for protein expression in mammalian cells(CHO cells).

• Purification batch purification using bench top.

• Candidate should have foundational experience in purification and QC

Nice to haves/preferred:

• Experience with high-throughput systems and automation.

• Experience with batch purification using a variety of scaffolds and basic QC.

• Industry experience is preferred.

Software skills:

• Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.

• Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.

Skills/techniques needed:

• Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.

• Strong communication, organizational, and interpersonal skills.

• Able to maintain a “can-do” attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs.

• Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials

• Able to troubleshoot cell culture system and think critically about experiments.

• Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work.

Responsibilities:

Department summary:

We are on a quest for cures and are committed to be the world’s premier, most research-intensive biopharmaceutical company. We identify and target steps in disease mechanisms or pathways that could be inhibited or enhanced. Our goal is to isolate a compound that is effective against a disease target. Using innovative thinking, state-of-the-art facilities and robust scientific methodology we collaborate to discover the next medical breakthrough.

Our teams combine leading drug discovery capabilities and world-class R&D with the purpose of turning breakthrough science into life-changing medicines.

• The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, Multispecific scaffolds, and novel protein modalities to enable all aspects of Early Discovery.

• Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.

• Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.

Job responsibilities in detail:

• Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.

• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.

• Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.

• Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.

• Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.

• Maintain and troubleshoot mammalian cell culture systems.

• Document protein production processes, prepare reports and communicate results

• ELN documentation.

Awards and Accolades:

America's Most Honored Businesses (Top 10%)

Awarded by USPAAC for the Fastest Growing Business in the US

12th Fastest Growing Staffing Company in USA by Staffing industry Analysts in the US (2020, 2019, 2020)

Fastest 50 by NJ Biz (2020, 2019, 2020)

INC 5000 Fastest growing for 8 consecutive years in a row (only 1.26% companies make it to this list)

Top 100 by Dallas Business Journal (2020 and 2019)

Proven Supplier of the Year by Workforce Logiq (2020 and 2019)

2019 Spirit of Alliance Award by Agile1

2018 Best of the Best Platinum Award by Agile1

2018 TechServe Alliance Excellence Awards Winner

2017 Best of the Best Gold Award by Agile1(Act1 Group)