Research Lab Tech I NEUROLOGY rl

The General Hospital Corporation; Boston (MA); Presencial; USD 52.000 - 76.000; Tempo integral

Resumo da vaga

Mass General Brigham's Ann Romney Center for Neurologic Diseases is seeking a Research Lab Tech I to investigate microbiome and immune mechanisms in neurologic disease models. You will administer treatments to mice, characterize behavior and pathology, and perform flow cytometry, ELISAs, DNA/RNA work, and bacterial culturing under supervision.

An ideal candidate has some lab experience; on-the-job training will be provided.

Qualificações

• Bachelor's degree in a related field is required.
• 1-2 years of laboratory experience preferred.
• Experience with laboratory procedures and working with mice is a plus.

Responsabilidades

• Administers bacteria or immunomodulatory agents to animal models and handles mice.
• Operates Flow Cytometry and performs ELISA, RNA/DNA work, and PCR.
• Maintains mouse colonies, records strains and breeding, and keeps lab notes updated.
• Cultures bacteria from the microbiome and prepares samples per protocol.
• Maintains supplies and performs routine data recording and analysis.

Conhecimentos

Lab procedures

Mice handling

Record keeping

Safety procedures

Team work

Formação

Bachelor's Degree in Related Field

Ferramentas

Flow Cytometry

ELISA

DNA extraction

PCR

Descrição da vaga

Site: The Brigham and Women's Hospital, Inc.

Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

Job Summary

The Ann Romney Center for Neurologic Diseases is seeking to hire a highly qualified and motivated Research Lab Tech I to investigate the role of the microbiome and the immune system in models of neurologic disease. Working under general supervision of Rafael Rezende, the Tech will execute protocols involving a variety of routine and non-routine experiments at a professional level, including but not exclusively involving administering treatments to animal models of neurologic disease, characterizing behavior and pathology in animal models, performing flow cytometry, ELISAs, DNA extraction, PCR, and culturing bacteria. The Research Lab Tech will record test results in an orderly fashion and maintain accurate laboratory notes as well as maintain laboratory supplies.

An ideal candidate will have some experience with laboratory procedures and working with mice. In addition, training will be provided on the job for specific procedures.

Does this position require Patient Care? No

Essential Functions

• As directed, administers bacteria or immunomodulatory agents to animal models of neurologic diseases. Will be required to handle mice including treatment administration (intraperitoneal, subcutaneous, and intravenous injections) and behavioral tests.
• Will be required to learn to operate Flow Cytometry machinery.
• Will be required to perform routine immunology techniques such as ELISA, western blot and basic procedures on laboratory animals; and molecular biology techniques like RNA purification, DNA purification and cloning.
• Will be required to maintain mouse colonies and to keep record of mouse strains and breeding.
• Will be required to perform cell culture, including culture and maintenance of various cell lines and hybridomas.
• Will be required to cultures bacteria from the microbiome and maintains stocks.
• Performs routine molecular experiments, including DNA extraction, and 16S rRNA amplicon library preparation.
• Maintains and orders supplies when needed. Assists with cleaning and maintaining the laboratory area in a neat and orderly condition.
• Maintains laboratory notebook of daily activities including all experiments and procedures.
• Conducts literature searches and review, compiling information from journals and suggesting uses of new techniques of research and laboratory techniques, consulting with other researchers and techniques to exchange information.
• Attends courses in safety procedures for the handling, storage and disposal of biohazardous, infectious, radioactive and toxic substances, ensuring that all laboratory activities are carried out in compliance with such procedures.
• Perform laboratory experiments following established protocols and procedures.
• Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines.
• Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed.
• Record experimental data accurately and maintain organized records of procedures, protocols, and results.
• Compile and analyze data using appropriate statistical and analytical methods.
• Assist in data interpretation and presentation, contributing to research findings and reports.
• Performs other duties as assigned or as position requires.

Qualifications

Education Bachelor's Degree Related Field of Study required

Can this role accept experience in lieu of a degree? Yes

Experience Prior laboratory experience, either through academic coursework, internships, or employment 1-2 years preferred

Knowledge, Skills and Abilities

• Must have a sound understanding of laboratory practices and procedures, usually acquired through one to three months on the job training.
• Ability to manage work processes in a neat and orderly way, to follow instructions, and to work both independently and collaboratively as a member of a team.
• Ability to multi-task and prioritize work with attention to detail and punctuality.
• On