Research Associate/Senior Research Associate, Cell Engineering

Colossal Biosciences; Dallas (TX); On-site; USD 65,000 - 90,000; Full time

Benefits offered by this job

Medical, dental, and vision coverage

Excellent paid time off and companyhol

Flexible spending accounts (FSA)

Company matched 401k retirement plan

Paid parental leave at 100% salary for

Job summary

Colossal Biosciences is seeking a talented Research Associate in cell biology for work on derivation, characterization and gene editing in non-model mammalian organisms. The role is on-site in Dallas, TX with relocation assistance available.

You will derive and characterize stable cell lines, optimize culture conditions, and contribute to genome editing workflows while collaborating with a multidisciplinary team to advance milestones and present findings to the group.

Qualifications

• Derive and characterize stable cell lines from primary tissues.
• Optimize cell culture conditions and delivery methods for gene editing.
• Maintain high-quality lab records and reports.

Responsibilities

• Derive and characterize stable cell lines from primary tissues.
• Microdissection for cell line derivations.
• Test novel cell culture conditions and delivery methods to optimize gene editing.
• Expand clonal lines for cryopreservation, genotyping and QC.
• Interrogate targeting, genotyping and QC workflows with team.
• Assist in making edited lines meeting QC for downstream SCNT.
• Plan, analyze and execute experiments to meet milestones.
• Prepare progress reports and present findings.
• Maintain organized lab records and documentation.

Skills

Primary cell line derivation

Culture optimization

Cryopreservation

Electroporation/transfection

Immunostaining

Flow cytometry

Aseptic technique

Multitasking

Scientific communication

Education

BA/BS/MA/MS in biological sciences or related field

Tools

None specified

Job description

An affiliate of Colossal is looking for a talented research associate in cell biology with an interest in the derivation, characterization, and gene editing in non-model mammalian organisms. You will work closely with other lab team members in efforts to innovate on protocols of genome editing, reprogramming and lineage differentiation using cutting edge technologies.

This position is based on-site in Dallas, TX. Relocation assistance is available.

Duties and Responsibilities

• Derive and characterize stable cell lines from primary tissues
• Microdissection skills for cell line derivations
• Test novel cell culture conditions and delivery methods to optimize gene editing
• Expand clonal cell lines for cryopreservation, genotyping and QC assays
• Work with team members to interrogate targeting, genotyping and QC workflows
• Assist with making edited cell lines that meet quality control criteria for downstream SCNT
• Schedule, analyze and execute experiments to deliver on project milestones
• Attend lab meetings, prepares reports and presentations on the progress of projects
• Demonstrate great organizational skills and excellent laboratory record maintenance

Required Skills and Abilities

• Experience in primary cell line derivation, culture optimization and cryopreservation
• Experience with electroporation and transfection to maximize gene delivery and viability
• Experience immunostaining cells for flow cytometry and fluorescent microscopy
• Exceptional aseptic technique as antibiotic free cultures are standard
• Organized and comfortable multi-tasking to deliver on concurrent projects on time
• Strong written and oral communication skills, as well as creative problem-solving skills

Preferred Skills and Abilities

• Experience with high throughput imaging platforms and data analysis
• Experience with automated cell culture systems
• Experience with stem cell culture, such as mouse embryonic stem cells (mESC) or induced pluripotent stem cells (iPSC)

Education and Experience

• BA/BS/MA/MS in biological sciences, bioengineering, molecular biology, genetics, or any science related field.

What Colossal Offers Full-Time Employees

• Medical, dental, and vision coverage
• Excellent paid time off and company holidays so you can rest and recharge
• Flexible spending accounts (FSA)
• Company matched 401k retirement plan
• Paid parental leave at 100% salary for up to 12 weeks
• Education reimbursement
• An opportunity to help us return the Earth to a healthier state

The team at Colossal is committed, colorful, passionate, intelligent, collaborative, creative, experienced, scientific and world-class. That sounds like a lot to fit into one building. That’s why we are spread out in several locations that include laboratories, test facilities, academic institutions, libraries and offices.

Through it all, however, we maintain a culture of positivity, hope, happiness and belief that we’re doing something big. Something Colossal.

Colossal is proud to be an equal employment opportunity workplace. We welcome people from all backgrounds as well as celebrate and support diversity. We are committed to providing an inclusive environment of mutual respect that is free from discrimination and harassment so everyone can thrive.

EEO is the Law