Recombinant Protein Purification Scientist II

Pfizer; Cambridge, Northern (MA, KY); Hybrid; USD 50,000 - 56,000; Full time

Job summary

Pfizer in Cambridge, MA is seeking a motivated bench scientist to join a biotherapeutics research team. This hands-on role focuses on recombinant protein expression, purification, and characterization to support downstream biotherapeutic research.

You will perform routine purification using FPLC/AKTA, assist with protein expression workflows, and help with labeling and characterization. Collaboration and meticulous record-keeping are essential in a fast-paced environment.

Qualifications

• Bachelor's degree in Biochemistry, Biology, Chemistry, Molecular Biology, or a related scientific discipline.
• At least 1 year of hands-on experience with recombinant protein purification.
• Hands-on experience with AKTA/FPLC or comparable chromatography systems.
• Understanding of protein purification and characterization principles.
• Strong laboratory organization and time-management skills.
• Ability to work effectively in a collaborative, fast-paced research environment.

Responsibilities

• Perform routine recombinant protein purification using FPLC/AKTA and related chromatography techniques.
• Support the production of recombinant proteins and antigens for downstream biotherapeutic research.
• Participate in protein expression workflows and molecular biology/DNA-related activities as needed.
• Perform protein characterization and assess protein quality, purity, concentration, and suitability for downstream applications.
• Work with antigens, antibodies, mAbs, Fabs, and other recombinant proteins.
• Perform routine protein labeling and conjugation, including fluorescent and biotin labeling.
• Assist with analytical techniques such as SDS-PAGE, Western blotting, and HPLC.
• Maintain accurate laboratory records, sample tracking, and experimental documentation.
• Manage multiple protein-production and purification requests while meeting project timelines.
• Work collaboratively with scientists across the research organization and provide purified proteins for downstream studies.
• Maintain a clean, organized, and compliant laboratory environment and follow all required laboratory safety and PPE procedures.

Skills

Time management

Lab organization

Collaborative work

Attention to detail

Education

Bachelor's degree in Biochemistry or related

Master's degree (preferred)

Tools

AKTA/FPLC

Job description

Pfizer in Cambridge, MA is seeking a motivated bench scientist to join a biotherapeutics research team. This hands-on role focuses on recombinant protein expression, purification, and characterization to support downstream biotherapeutic research.

You will perform routine purification using FPLC/AKTA, assist with protein expression workflows, and help with labeling and characterization. Collaboration and meticulous record-keeping are essential in a fast-paced environment.