Senior Scientist, Protein Production & Purification

Doist; Andover (MA); On-site; USD 125,000 - 175,000; Full time

Job summary

Generate:Biomedicines is seeking a Senior Scientist I in Andover, MA to independently lead complex protein production, purification, and characterization efforts supporting drug discovery and platform initiatives. You will define technical approaches, integrate data across workflows, and drive scalable, data-rich workflows across multiple protein modalities.

You will serve as a technical contact for purification and characterization strategy, partnering with cross-functional teams and external

Qualifications

• Independently define, plan, and execute complex protein production, purification, and characterization strategies across multiple discovery and platform initiatives.
• Establish technical milestones, decision criteria, dependencies, and risk-mitigation plans.
• Lead the design, optimization, scale-up, and troubleshooting of protein expression workflows, including transient mammalian and cell-free protein synthesis platforms.
• Provide technical input into construct design, molecular biology, and stable-expression activities as needed.
• Lead purification workflow development from capture through polishing, with emphasis on larger-scale or later-stage production.
• Design and interpret chromatography screening studies, including predictor-plate approaches, resin selection, operating-condition optimization, and scale translation.
• Produce high-quality protein material across a range of formats and scales.
• Partner with downstream stakeholders to establish fit-for-purpose quality attributes and acceptance criteria based on intended use.
• Select, apply, and interpret analytical and biochemical characterization methods to assess protein purity, identity, concentration, oligomeric state, aggregation, stability, etc.
• Integrate expression, purification, and characterization data to identify root causes, resolve complex technical challenges, evaluate trade-offs, and recommend next steps.
• Clearly communicate the evidence, assumptions, risks, and uncertainty supporting technical decisions.
• Serve as a technical point of contact for protein production, purification, and characterization strategy.
• Influence cross-functional decisions by translating scientific findings into clear recommendations for discovery, platform, analytical, and data-focused partners.
• Lead technical interactions with CROs, CDMOs, vendors, and other external partners as appropriate.
• Define technical work packages, support method or process transfer, review data, troubleshoot issues, and ensure deliverables meet scientific requirements.
• Drive the development of scalable, reproducible, and well-documented workflows.
• Advance automation, high-throughput approaches, structured experimental data, and AI/ML-enabled tools to improve productivity.
• Develop and share new methods, technical standards, and best practices.
• Advise and mentor colleagues, provide constructive scientific feedback, and contribute to an inclusive, collaborative team.
• Demonstrate accountability for scientific quality and delivery by tracking progress and communicating outcomes clearly.

Responsibilities

• Independently define, plan, and execute complex protein production, purification, and characterization strategies across multiple discovery and platform initiatives.
• Establish milestones, decision criteria, dependencies, and risk-mitigation plans.
• Lead design, optimization, scale-up, and troubleshooting of protein expression workflows (mammalian and cell-free).
• Input into construct design, molecular biology, and stable-expression activities as needed.
• Lead purification workflow development from capture through polishing.
• Design chromatography screening studies and scale translation.
• Produce high-quality protein material across formats and scales.
• Partner to set fit-for-purpose QA criteria based on intended use.
• Select and interpret analytical methods for protein purity, identity, concentration, oligomeric state, and stability.
• Integrate expression, purification, and characterization data to identify root causes and inform next steps.
• Communicate evidence, risks, and uncertainties supporting decisions.
• Serve as technical contact for protein production strategy.
• Influence cross-functional decisions with data-driven recommendations.
• Lead interactions with CROs/CDMOs/vendors; define work packages and transfer.
• Drive scalable workflows; promote automation and AI/ML tools.
• Develop and share new methods, standards, and best practices.
• Mentor colleagues and share constructive scientific feedback.
• Track progress and transparently communicate successes and lessons learned.

Skills

Protein production

Protein purification

Protein characterization

Data analysis

Cross-functional collaboration

Education

Ph.D. in biochemistry or related

B.S./M.S. in relevant field with 12+ years

Tools

Chromatography

Mammalian expression

Cell-free expression

Data management systems

Job description

Generate:Biomedicines is seeking a Senior Scientist I in Andover, MA to independently lead complex protein production, purification, and characterization efforts supporting drug discovery and platform initiatives. You will define technical approaches, integrate data across workflows, and drive scalable, data-rich workflows across multiple protein modalities.

You will serve as a technical contact for purification and characterization strategy, partnering with cross-functional teams and external