Protein Expression Technician: Insect & Mammalian Cultures

Caltech; Pasadena (CA); Hybride; USD 28 000 - 37 000; Plein temps

Avantages offerts par ce poste

Health, dental, and vision insurance

Tuition reimbursement

Paid time off

Retirement plans

Résumé du poste

Caltech invites a Research Technician with extensive hands-on experience in insect cell culture (Sf9 and Hi5) using the baculovirus expression system, and mammalian cell culture across diverse lines. The successful candidate will support ongoing structural and functional studies of protein complexes by producing high-quality recombinant proteins in both insect and mammalian systems.

You will generate constitutive constructs, maintain cell cultures, produce baculoviruses, optimize expression,

Qualifications

• Bachelor’s degree in Biology, Biochemistry, Molecular Biology, or related field.
• 2 years of hands-on Sf9/Hi5 cell culture and baculovirus-based protein expression.
• Proficiency in aseptic technique and routine mammalian cell culture.
• Familiarity with restriction enzyme cloning, western blotting, SDS-PAGE, immunofluorescence microscopy, and protein purification.

Responsabilités

• Generate bacterial, insect cell, and mammalian expression constructs.
• Maintain and passage insect cells (Sf9/Hi5) and mammalian cell cultures.
• Generate and amplify recombinant baculoviruses for protein expression.
• Transfect and maintain mammalian cell lines for transient and stable expression.
• Optimize expression protocols for high-yield protein production.
• Perform small- and large-scale protein expression experiments.
• Harvest cells and assist with downstream purification.
• Perform immunofluorescence microscopy to assess localization.
• Conduct SDS-PAGE and western blotting as part of routine experiments.
• Maintain thorough records of experiments, protocols, and inventories.
• Participate in general lab maintenance and support researchers.

Connaissances

Aseptic technique

Mammalian cell culture

Restriction enzyme cloning

Western blotting

SDS-PAGE

Immunofluorescence microscopy

Protein purification

Team collaboration

Independent work

Formation

Bachelor’s degree in Biology, Biochemistry, Molecular Biology, or related field

Outils

Baculovirus expression system

Immunofluorescence microscope

Chromatography

Description du poste

Caltech invites a Research Technician with extensive hands-on experience in insect cell culture (Sf9 and Hi5) using the baculovirus expression system, and mammalian cell culture across diverse lines. The successful candidate will support ongoing structural and functional studies of protein complexes by producing high-quality recombinant proteins in both insect and mammalian systems.

You will generate constitutive constructs, maintain cell cultures, produce baculoviruses, optimize expression,