Platform Lead — Molecular Biology & Protein Expression

Eli Lilly and Company; San Diego (CA); On-site; USD 72,000 - 189,000; Full time

Benefits offered by this job

401(k) plan

Pension

Vacation benefits

Medical, dental, vision benefits

Flexible benefits

Life insurance

Time off and leave

Well-being programs

Job summary

Eli Lilly and Company in San Diego, CA seeks a Principal Scientist/Senior Principal Scientist in Molecular Biology & Protein Expression to drive a centralized platform, tackle challenging membrane protein targets, and lead automation initiatives.

You will design cloning strategies, express proteins in E. coli, insect and mammalian cells, mentor staff, document results in LIMS, and collaborate across teams to accelerate discovery.

Qualifications

• Bachelor- or master-level degree in a biological science with strong cloning and protein expression experience.
• Hands-on experience with multiple host systems and construct design for diverse targets.
• Excellent documentation, collaboration, and communication skills.

Responsibilities

• Design and execute cloning strategies and construct design for targets.
• Perform small- and large-scale expression in multi-host systems.
• Assess expression via SDS-PAGE, Western blot, SEC, and UV spectroscopy.
• Develop workflows, automate screening, and improve throughput.
• Mentor junior scientists and maintain rigorous data records in LIMS.

Skills

Molecular cloning

Protein expression

SDS-PAGE

Western blot

Analytical SEC

UV spectroscopy

Gibson assembly

Golden Gate

In-Fusion

Site-directed mutagenesis

E. coli expression

Baculovirus/insect cells

Mammalian expression

Education

B.S. or M.S. in Biological Sciences, Biochemistry, Molecular Biology, Chemistry, Biophysics, or related discipline

Tools

Gibson

Golden Gate

In-Fusion

Site-directed mutagenesis

AKTA FPLC

Benchling

LIMS

Job description

Eli Lilly and Company in San Diego, CA seeks a Principal Scientist/Senior Principal Scientist in Molecular Biology & Protein Expression to drive a centralized platform, tackle challenging membrane protein targets, and lead automation initiatives.

You will design cloning strategies, express proteins in E. coli, insect and mammalian cells, mentor staff, document results in LIMS, and collaborate across teams to accelerate discovery.