Oligonucleotide Synthesis & Conjugation Associate II

Crispr-Therapeutics-1; Boston (MA); On-site; USD 85,000 - 92,000; Full time

Job summary

CRISPR Therapeutics, Inc. is seeking a hands-on Research Associate II to support oligonucleotide synthesis, purification, and conjugation for gene editing programs. You will operate automated synthesizers, purify and characterize material, and prepare conjugates for tissue-targeted delivery, reporting to senior scientists.

The role emphasizes bench work, rigorous documentation, and collaboration within a fast-moving discovery chemistry group, with on-site work and strict safety practices.

Qualifications

• BS in Chemistry, Biochemistry, Chemical Biology, or a related discipline with 2+ years of relevant laboratory experience; or MS in a related discipline with 0–2+ years of experience.
• Hands-on experience with solid-phase oligonucleotide synthesis on an automated synthesizer, including cleavage, deprotection, and workup.
• Demonstrated experience with preparative HPLC purification and interpretation of analytical HPLC or LC-MS data.
• Practical experience with at least one bioconjugation chemistry, such as NHS ester, maleimide–thiol, or click chemistry, applied to oligonucleotides, peptides, or proteins.
• Strong general wet-chemistry experience, including accurate weighing, solution preparation, air- and moisture-sensitive handling, and anhydrous techniques.
• Meticulous documentation habits and comfort working in an electronic lab notebook.
• Ability to manage multiple parallel experiments and deliver against timelines in a fast-paced environment.

Responsibilities

• Oligonucleotide Synthesis: solid-phase synthesis on automated synthesizers; modifications and deprotection.
• Purification and Analysis: purify by RP-HPLC, IEX-HPLC; LC-MS and UV quantification.
• Conjugate preparation: prepare small molecule–oligonucleotide and antibody–oligonucleotide conjugates; assess DAR.
• Documentation and data communication: maintain electronic lab notebook records; present data at meetings.
• Safety and compliance: work on-site; follow EHS requirements and safe handling procedures.

Skills

Oligonucleotide synthesis

RP-HPLC purification

LC-MS data interpretation

Bioconjugation chemistry

Electronic lab notebook documentation

Education

Bachelor's degree in Chemistry, Biochemistry, Chemical Biology or related

Master's degree in related field

Tools

LC-MS deconvolution software

Empower

Chromeleon

Unicorn

HPLC instrumentation

Job description

CRISPR Therapeutics, Inc. is seeking a hands-on Research Associate II to support oligonucleotide synthesis, purification, and conjugation for gene editing programs. You will operate automated synthesizers, purify and characterize material, and prepare conjugates for tissue-targeted delivery, reporting to senior scientists.

The role emphasizes bench work, rigorous documentation, and collaboration within a fast-moving discovery chemistry group, with on-site work and strict safety practices.