Neuroscience Research Assistant — iPSC & Imaging

First Roles; Boston (MA); On-site; USD 29,000 - 40,000; Full time

Job summary

Mass General Brigham's The Brigham and Women's Hospital, Inc. is seeking a lab-focused candidate to support molecular biology and iPSC-derived neuron projects at 60 Fenwood Road.

The role involves routine experiments, data collection, and SOP development under supervision, with opportunities to contribute to epidemiological studies and population-based research. New graduates with relevant coursework and basic cell culture experience are encouraged to apply.

Qualifications

• New grad with some relevant coursework (0-1 year preferred).
• Good cell culture experience with human cells (HEK293 or immortalized line).
• iPSC stem cell culture experience is a plus but not required.
• Excellent cell culture practice/sterile techniques are necessary.
• Medium to high level of computer literacy and a basic understanding of statistical concepts are needed.
• Basic understanding of molecular techniques: cloning, western blot, RNA isolation, qPCR.

Responsibilities

• Maintenance, passaging, cataloguing, and culturing of iPSC cells.
• Differentiation iPSCs to neurons and glia and aging of these cultures.
• Maintenance of Attune flow cytometry and ordering the related reagents.
• Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope.
• Maintenance of HEK293 cells, generation of stable cell lines.
• Assistance with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps.
• Analysis of data from western blot / microscopy / flow cytometry.
• Generate SOPs for the lab and maintain lab notes (digital).
• Report daily to supervisor and weekly to PI; interact with lab members to troubleshoot.
• Share lab duties with other technicians; data collection, management, manipulation, analysis.

Skills

New grad

Cell culture

iPSC culture

Sterile techniques

Computational literacy

Molecular techniques

Education

Bachelor's Degree

Job description

Mass General Brigham's The Brigham and Women's Hospital, Inc. is seeking a lab-focused candidate to support molecular biology and iPSC-derived neuron projects at 60 Fenwood Road.

The role involves routine experiments, data collection, and SOP development under supervision, with opportunities to contribute to epidemiological studies and population-based research. New graduates with relevant coursework and basic cell culture experience are encouraged to apply.