Research Assistant I NEUROLOGY kl

Brigham and Women's Hospital; Boston (MA); On-site; USD 29,000 - 40,000; Full time

Job summary

Mass General Brigham seeks a lab associate to support iPSC culture, neuronal differentiation, and viability/toxicity assays. You will assist in coordinating research activities, data collection, and SOP development under supervision in the Khurana lab.

The role emphasizes meticulous lab practices, organization, and collaboration with a diverse team, including data management and outreach tasks. Remote work is not offered, and the position is onsite in Boston.

Qualifications

• Bachelor's Degree Science required
• Can this role accept experience in lieu of a degree? Yes
• New grad with some relevant coursework 0-1 year preferred
• Good cell culture experience with human cells (HEK293 or any other immortalized line)
• iPSC stem cell culture experience is a plus but not required
• Excellent cell culture practice/sterile techniques are necessary
• Medium to high level of computer literacy and a basic understanding of statistical concepts are needed
• Basic understanding of molecular techniques, microscopy, flow cytometry is desired
• Understanding of basic molecular concepts: molecular cloning, western blot, RNA isolation, qPCR, western blot is desired

Responsibilities

• Maintenance, passaging, cataloguing, and culturing of iPSC cells
• Differentiation of iPSCs to neurons and aging of these cultures
• Maintenance of Attune flow cytometry and ordering the related reagents
• Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope
• Maintenance of HEK293 cells, generation of stable cell lines
• Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps
• Analysis of data from western blot/ microscopy/ flow cytometry
• Lab note-keeping and SOPs for the lab
• Excellent organizational skills, labeling of experiments, file management
• Report daily to supervisor and weekly to PI
• Interact with lab members to troubleshoot experiments
• Share lab duties with other technicians
• Data collection, management, manipulation, analysis, and reporting
• Study cohort management
• Outreach communication with subjects, mailings, and obtaining documents
• Assists with epidemiological studies and population-based research

Skills

Cell culture experience

iPSC culture

Molecular cloning basics

Flow cytometry understanding

Data analysis basics

Communication skills

Education

Bachelor's Degree in Science

Tools

Attune flow cytometer

Incucyte

Nikon Biostation

Gibson assembly

Gateway cloning

Job description

Job Summary

Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham.

Summary

The candidate will work independently under the supervision of Vikram Khurana, MD, PhD, and a senior researcher in the Khurana lab (instructor/assistant professor). Depending on the specific project needs and candidate skill set, the role will range from molecular biology focused bench work to development and optimization of assays for viability/toxicity in iPSC (induced pluripotent stem cells) derived neurons. These assays will include techniques such as flow cytometry, longitudinal imaging of neurons, plate-based live cell and calorimetric assays and western blotting. The candidate is expected to be diligent, highly organized, and collegial.

The position provides assistance to the Principal Investigator and Study Team in coordinating research activities that may include conducting a variety of routine experiments with established methodologies, data collection and management, and other research activities as assigned.

Candidates who are in the process of completing their bachelor's degree have a grace period of six months from their hire date (up to 1 year if starting per diem)to provide degree equivalency verification.

Does this position require Patient Care? No

Essential Functions

• Maintenance, passaging, cataloguing, and culturing of iPSC cells.
• Differentiation iPSCs to neurons and glia and aging of these cultures.
• Maintenance of Attune flow cytometry and ordering the related reagents
• Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope.
• Maintenance of HEK293 cells, generation of stable cell lines.
• Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps.
• Analysis of data from western blot/ microscopy/ flow cytometry.
• The candidate will have excellent lab note-keeping habits, mostly digital(LabArchieves) and will generate Standard Operating Procedures (SOPs) for the lab.
• The candidate will have excellent organizational skills, labeling of experiments, file management.
• The candidate will report daily to the immediate supervisor and weekly to the Principal Investigator.
• The candidate will interact with junior and senior members of the lab to troubleshoot experiments.
• The candidate will share lab duties with other technicians.
• Responsible for data collection, management, manipulation, analysis, and reporting.
• Study cohort management.
• Outreach communication with subjects, conducting mailings, follow-up on mailings, and obtaining necessary documents.
• Provides assistance on work related to epidemiological studies and other population-based research.

Qualifications

Education

Bachelor's Degree Science required

Can this role accept experience in lieu of a degree? Yes

Experience and Qualifications

• New grad with some relevant coursework 0-1 year preferred
• Good cell culture experience with human cells (HEK293 or any other immortalized line).
• iPSC stem cell culture experience is a plus but not required.
• Excellent cell culture practice/sterile techniques are necessary.
• Medium to high level of computer literacy and a basic understanding of statistical concepts are needed.
• Basic understanding of molecular techniques, microscopy, flow cytometry is desired.
• Understanding of basic molecular concepts: molecular cloning, western blot, RNA isolation, qPCR, western blot is desired.

Knowledge, Skills and Abilities

• Excellent oral and written communication skills.
• Strong interpersonal skills are required for working in a highly diverse lab.
• Excellent organizational skills and ability to prioritize a variety of tasks.
• Excellent data management skills and attention to detail.
• Good presentation skills are a plus.
• Computer literacy, including database tools.
• Ability to follow directions and exhibit professionalism.

Additional Job Details (if applicable)

• Remote Type: Onsite
• Work Location: 60 Fenwood Road
• Scheduled Weekly Hours: 40
• Employee Type: Regular
• Work Shift: Day (United States of America)
• Pay Range: $21.00 - $29.01/Hourly
• Grade: 5

At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth.

EEO Statement: 2200 The Brigham and Women's Hospital, Inc. is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected under Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran's Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at (857)-282-7642.

Mass General Brigham Competency Framework. At Mass General Brigham, our competency framework defines what effective leadership "looks like" by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline.