Neuroscience Research Assistant - iPSC & Cell Culture

Brigham and Women's Hospital; Boston (MA); On-site; USD 29,000 - 40,000; Full time

Job summary

Mass General Brigham seeks a lab associate to support iPSC culture, neuronal differentiation, and viability/toxicity assays. You will assist in coordinating research activities, data collection, and SOP development under supervision in the Khurana lab.

The role emphasizes meticulous lab practices, organization, and collaboration with a diverse team, including data management and outreach tasks. Remote work is not offered, and the position is onsite in Boston.

Qualifications

• Bachelor's Degree Science required
• Can this role accept experience in lieu of a degree? Yes
• New grad with some relevant coursework 0-1 year preferred
• Good cell culture experience with human cells (HEK293 or any other immortalized line)
• iPSC stem cell culture experience is a plus but not required
• Excellent cell culture practice/sterile techniques are necessary
• Medium to high level of computer literacy and a basic understanding of statistical concepts are needed
• Basic understanding of molecular techniques, microscopy, flow cytometry is desired
• Understanding of basic molecular concepts: molecular cloning, western blot, RNA isolation, qPCR, western blot is desired

Responsibilities

• Maintenance, passaging, cataloguing, and culturing of iPSC cells
• Differentiation of iPSCs to neurons and aging of these cultures
• Maintenance of Attune flow cytometry and ordering the related reagents
• Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope
• Maintenance of HEK293 cells, generation of stable cell lines
• Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps
• Analysis of data from western blot/ microscopy/ flow cytometry
• Lab note-keeping and SOPs for the lab
• Excellent organizational skills, labeling of experiments, file management
• Report daily to supervisor and weekly to PI
• Interact with lab members to troubleshoot experiments
• Share lab duties with other technicians
• Data collection, management, manipulation, analysis, and reporting
• Study cohort management
• Outreach communication with subjects, mailings, and obtaining documents
• Assists with epidemiological studies and population-based research

Skills

Cell culture experience

iPSC culture

Molecular cloning basics

Flow cytometry understanding

Data analysis basics

Communication skills

Education

Bachelor's Degree in Science

Tools

Attune flow cytometer

Incucyte

Nikon Biostation

Gibson assembly

Gateway cloning

Job description

Mass General Brigham seeks a lab associate to support iPSC culture, neuronal differentiation, and viability/toxicity assays. You will assist in coordinating research activities, data collection, and SOP development under supervision in the Khurana lab.

The role emphasizes meticulous lab practices, organization, and collaboration with a diverse team, including data management and outreach tasks. Remote work is not offered, and the position is onsite in Boston.