Multi-Specifics Expression Scientist (CHO, High-Throughput)

BioSpace; North Chicago (IL); On-site; USD 120,000 - 180,000; Full time

Job summary

AbbVie Biotherapeutics and Genetic Medicine seeks an experienced protein expression scientist to join a fast-paced CHO cell line engineering group. You will focus on expressing multi‑specific biologics, validating stable CHO lines, and enabling scalable workflows for drug discovery.

Responsibilities include optimizing titer, monomer ratio, and chain pairing; leveraging automation and high‑throughput methods, and contributing to data digitization and reporting.

Qualifications

• Bachelor's or Master's in biology, chemistry, biochemistry or related field with typically 5+ (BS) or 2+ (MS) years of experience.
• Hands‑on experience with cell culture and expression of monoclonal antibodies, bi‑specifics and multi‑specifics in CHO cells lines including thaw, culture, expression and cryopreservation.
• Prior experience in generation of stable CHO cell lines expressing complex multi‑specific molecules.

Responsibilities

• Expression of novel multi‑specific biologics formats to support therapeutic area drug discovery projects.
• Generation and screening of stable CHO cell lines with optimized attributes (i.e. titer, monomer, chain‑pairing) using 2 or more vectors.
• Determine optimal chain ratio for 3‑ and 4‑chain molecules in CHO cells using multiple selection strategies and process optimization.
• Process development and optimization for batch and fed‑batch bioreactor and shake flask expressions of stable CHO cell lines expressing complex multi‑chain molecules.
• Characterization of stable CHO line copy number and mRNA transcript levels using ddPCR and related molecular tools.
• Apply targeted integration technologies using screening workflows, including FACS‑based single‑cell sorting to generate mini pools.

Skills

Cell culture

Protein expression

Mammalian expression

FACS

ddPCR

Automation

Education

Bachelor's degree in biology

Master's degree in biochemistry

Tools

Wave bioreactors

Job description

AbbVie Biotherapeutics and Genetic Medicine seeks an experienced protein expression scientist to join a fast-paced CHO cell line engineering group. You will focus on expressing multi‑specific biologics, validating stable CHO lines, and enabling scalable workflows for drug discovery.

Responsibilities include optimizing titer, monomer ratio, and chain pairing; leveraging automation and high‑throughput methods, and contributing to data digitization and reporting.