Scientist I MultiSpecifics Expression

AbbVie; North Chicago (IL); On-site; USD 110,000 - 160,000; Full time

Job summary

AbbVie Biotherapeutics and Genetic Medicine is seeking a qualified scientist to advance discovery programs through expression and characterization of complex multi-specific biologics. The role involves CHO cell line generation, process development in bioreactors, and implementing high-throughput workflows.

Candidate will analyze data, troubleshoot expression challenges, and contribute to purification and biophysical characterization using HPLC.

Qualifications

• Bachelor’s or Master’s in biology/biochemistry or related field with 5+ (BS) or 2+ (MS) years of experience.
• Hands-on experience with cell culture and expression of monoclonal antibodies, bi-specifics and multi-specifics in CHO cells lines.
• Experience with targeted integration, automation, and analytical characterization of multi-specific proteins desirable.

Responsibilities

• Express novel multi-specific biologics formats to support drug discovery projects.
• Generate and screen stable CHO cell lines with optimized attributes.
• Optimize chain ratios for 3- and 4-chain molecules in CHO cells.
• Develop and optimize expression of stables CHO lines in bioreactors and shake flasks.
• Characterize CHO line copy number and mRNA levels using ddPCR and related tools.
• Apply screening workflows including FACS-based sorting to generate mini pools.
• Leverage automation platforms and high-throughput workflows to improve efficiency.
• Contribute to data digitization, analysis, and reporting.

Skills

Cell culture

Monoclonal antibodies

Bi-specifics

Multi-specific molecules

Problem solving

Education

Bachelors or Master’s in biology/biochemistry or related field

Tools

Wave bioreactors

ddPCR

FACS

Analytical HPLC

Job description

Company Description

About AbbVie

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas including immunology, oncology and neuroscience - and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at www.abbvie.com. Follow @abbvie on LinkedIn, Facebook, Instagram, X and YouTube.

Job Description

Overview:

AbbVie Biotherapeutics and Genetic Medicine is an integrated, global discovery organization responsible for biotherapeutics drug research across disease areas including immunology, oncology, and neuroscience. Our scope includes the discovery of innovative biologic medicines from the early exploratory stage and their support through clinical development. We are growing a world-class team of scientists and leaders who will drive the discovery and advancement of innovative biologic-based therapies. Careers in AbbVie Biotherapeutics and Genetic Medicine provide unique opportunities to work with cross-functional teams that drive exceptional science and deliver innovative medicines to patients. We are looking for diverse candidates with strong scientific and leadership experience to join the team and can offer exciting and rewarding career opportunities for these candidates.

Key Responsibilities:

• Expression of novel multi-specific biologics formats to support therapeutic area drug discovery projects.
• Generation and screening of stable CHO cell lines with optimized attributes (i.e. titer, monomer, chain-pairing) using 2 or more vectors.
• Determine optimal chain ratio for 3- and 4-chain molecules in CHO cells using multiple selection strategies and process optimization.
• Process development and optimization for batch and fed-batch bioreactor and shake flask expressions of stable CHO cell lines expressing complex multi-chain molecules.
• Characterization of stable CHO line copy number and mRNA transcript levels using ddPCR and related molecular tools.
• Apply targeted integration technologies using screening workflows, including FACS-based single-cell sorting to generate mini pools.
• Implement and leverage automation platforms and high-throughput workflows to increase efficiency.
• Contribute to data digitization efforts, including electronic data capture, analysis, and reporting.
• Troubleshoot challenges related to expression, stability, glycosylation, aggregation, and chain pairing.
• Purification and biophysical characterization of analytical scale expression samples, using analytical HPLC to evaluate titer, monomer, and chain pairing efficiency.
• Work independently on the design, execution, and interpretation of experimental results.
• Analyze, document, and report data concisely and rigorously.
• Handling stable CHO pools and cell lines including thaw, culture, expression and cryopreservation.

Qualifications

Qualifications:

Position will be hired based on level of experience and education

• Bachelors or Master’s in biology, chemistry, biochemistry or related field with typically 5+ (BS) or 2+ (MS) years of experience.
• Hands-on experience with cell culture and expression of monoclonal antibodies, bi-specifics and multi-specifics in CHO cells lines including thaw, culture, expression and cryopreservation.
• Prior experience in generation of stable CHO cell lines expressing complex multi-specific molecules.
• Prior experience in process development, process optimization, and automation.
• Experience with analytical characterization of multi-specifics desirable.
• Experience with targeted integration, FACS, and ddPCR desirable.
• Demonstrated proficiency in vector design is desirable.
• Prior experience in mammalian expression using Wave bioreactors desirable.
• Demonstrated proficiency in overcoming problems (i.e. poor chain pairing, low expression, aggregation, glycosylation) with challenging therapeutic multi-specific proteins desirable.

Additional Information

• The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this roleat the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on manyfactors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future.
• We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees.
• This job is eligible to participate in our short-term incentive programs.

Note: No amount of pay is considered to be wages or compensation until such amount isearned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that areallocable to a particular employee remains in the Company’s sole and absolute discretion unless and until paid and may be modified at the Company’s sole and absolutediscretion, consistent with applicable law.

AbbVie is an equal opportunity employer and is committed to operating with integrity, driving innovation, transforming lives and serving our community. Equal Opportunity Employer/Veterans/Disabled.

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