Molecular Biology Research Associate – Tissue RNA & RT-qPCR

Yoh, A Day & Zimmermann Company

South San Francisco (CA)

On-site

USD 48,000 - 62,000

Full time

2 days ago

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Job summary

Yoh, a Day & Zimmermann company, is seeking a Research Associate I/II in South San Francisco to support molecular biology and ex vivo studies, focusing on RNA extraction from tissue and expression analysis by RT-qPCR.

The ideal candidate has hands-on experience with phenol-chloroform RNA extraction (TRIzol/TRI Reagent/QIAzol) and 384‑well RT-qPCR, is comfortable working onsite, and can generate accurate, reproducible data under fast-paced conditions.

Qualifications

Bachelor's degree in biology or related life science field.
Hands-on experience with phenol-chloroform RNA extraction from animal tissues.
Experience with RT-qPCR gene-expression analysis using 384-well plates.
Ability to follow SOPs and manage multiple experiments with minimal supervision.

Responsibilities

Independently process animal tissue samples, including tissue preparation and homogenization, followed by phenol‑chloroform RNA extraction.
Perform RNA quantification and quality assessment, cDNA synthesis, and RT‑qPCR gene‑expression analysis.
Prepare and run 384‑well RT‑qPCR plates using accurate pipetting, appropriate controls, reference genes, and established protocols.
Analyze gene‑expression data, evaluate assay quality, interpret experimental results, and troubleshoot technical variability.
Perform molecular and biomarker assays using biological samples such as tissue lysates, serum, plasma, or cell-derived materials.
Maintain mammalian cell cultures using aseptic techniques, including routine passaging, counting, and culture maintenance.
Support ex vivo research through tissue processing and primary cell preparation or isolation.
Execute established laboratory protocols efficiently while managing multiple experiments and sample sets.
Maintain accurate experimental documentation, track samples, and communicate results to research teams.