Molecular Biology Associate, Tissue RNA & RT-qPCR Onsite

Yoh, A Day & Zimmermann Company; San Francisco (CA); On-site; USD 48,000 - 62,000; Full time

Benefits offered by this job

Medical benefits

Health Savings Account (HSA)

Life & Disability Insurance

Voluntary benefits

EAP

401K Retirement Savings Plan

Direct Deposit & weekly epayroll

Referral bonuses

Certification and training

Job summary

Yoh, a Day & Zimmermann company, is seeking a Research Associate I/II to support molecular biology and ex vivo research programs at a growing biotechnology company in South San Francisco, CA. The role focuses on tissue RNA extraction and RT-qPCR workflows, using phenol-chloroform methods and 384-well formats, with an emphasis on accuracy and reproducibility.

The successful candidate will perform RNA extraction, qPCR, and biomarker assays, maintain documentation, and contribute to multiple

Qualifications

• Bachelor's degree in Biology, Molecular Biology, Biochemistry, Cell Biology, or related life science discipline.
• Direct hands-on lab experience in molecular biology and ex vivo research environments.
• Experience performing phenol-chloroform RNA extraction from animal tissues using TRIzol/TRI Reagent/QIAzol or equivalent.
• Experience performing RT-qPCR gene-expression analysis using 384-well plates.
• Experience RNA quality assessment, cDNA synthesis, assay setup, and data analysis.
• Strong pipetting skills for high-throughput multiwell assays.
• Independent follow SOPs and maintain accurate records.

Responsibilities

• Independently process animal tissue samples, including tissue prep and homogenization, then RNA extraction.
• Perform RNA quantification, quality assessment, cDNA synthesis, and RT-qPCR gene-expression analysis.
• Prepare and run 384-well RT-qPCR plates with proper controls and reference genes.
• Analyze gene-expression data, evaluate assay quality, and troubleshoot variability.
• Conduct molecular and biomarker assays on tissue lysates, serum, plasma, or cell-derived materials.
• Maintain mammalian cell cultures using aseptic techniques and routine passaging.
• Support ex vivo research through tissue processing and primary cell preparation.
• Execute established protocols efficiently while managing multiple experiments.
• Maintain detailed experimental documentation and track samples.

Skills

RNA extraction

RT-qPCR

Mammalian cell culture

Phenol-chloroform extraction

qPCR data analysis

High-throughput assays

Education

Bachelor's degree in Biology or related life science

Tools

GraphPad Prism

Excel

Electronic lab notebooks

Job description

Yoh, a Day & Zimmermann company, is seeking a Research Associate I/II to support molecular biology and ex vivo research programs at a growing biotechnology company in South San Francisco, CA. The role focuses on tissue RNA extraction and RT-qPCR workflows, using phenol-chloroform methods and 384-well formats, with an emphasis on accuracy and reproducibility.

The successful candidate will perform RNA extraction, qPCR, and biomarker assays, maintain documentation, and contribute to multiple