iPSC & NMJ Research Assistant — Flexible Schedule

Boston Children's Hospital; Boston (MA); On-site; USD 55,000 - 75,000; Full time

Benefits offered by this job

Flexible schedules

Health insurance

Retirement plan

Job summary

Boston Children's Hospital invites applications for a Laboratory Research Assistant I in the Eagles Lab at Harvard Medical School. The role focuses on cutting-edge neuromuscular disease research using human iPSCs, spinal cord organoids, skeletal muscle progenitors, and NMJ models to study SMA and potential therapies.

The successful candidate will contribute to culture, differentiation, and functional assays.

Qualifications

• Bachelor's degree in STEM or Psychology required.
• Experience with human iPSC or stem cell culture highly preferred.
• Experience with immunostaining and microscopy is desirable.
• Ability to maintain aseptic cell culture workflows and meticulous lab notes.
• Willingness to work evenings/weekends as needed.

Responsibilities

• Culture human iPSCs and expand/cryopreserve lines.
• Differentiate iPSCs into spinal cord organoids and myogenic progenitors.
• Establish and maintain NMJ cultures and assembloids.
• Prepare and execute NMJ drug screens and hit-validation experiments.
• Perform microscopy and live-cell imaging (fluorescence, confocal, GCaMP).
• RNA purification, cDNA prep, and qPCR.
• Dissociate cells, perform FACS, and prepare samples for single-cell profiling.
• Assist with mouse work (genotyping, tissue collection).
• Maintain media/reagents and lab equipment; document experiments carefully.

Skills

iPSC culture

Organoid culture

Immunostaining

Microscopy

qPCR

FACS

Live-cell imaging

Mammalian cell culture

Education

Bachelor's degree in STEM or Psychology

Biology/Neuroscience background preferred

Tools

Confocal microscopy

FACS

qPCR instruments

Job description

Boston Children's Hospital invites applications for a Laboratory Research Assistant I in the Eagles Lab at Harvard Medical School. The role focuses on cutting-edge neuromuscular disease research using human iPSCs, spinal cord organoids, skeletal muscle progenitors, and NMJ models to study SMA and potential therapies.

The successful candidate will contribute to culture, differentiation, and functional assays.