Immunology Discovery Scientist: Cell-Based Assays

Eli Lilly and Company; Indianapolis (IN); On-site; USD 66,000 - 172,000; Full time

Benefits offered by this job

Company bonus

401(k) plan

Vacation benefits

Comprehensive medical/dental/vision

Job summary

Eli Lilly and Company in Indianapolis, IN, is seeking a scientist to design and execute immunology cell-based assays and analyze preclinical data supporting novel therapeutics. You will work with primary human, monkey, and mouse lymphocytes, applying multi-parameter flow cytometry, qPCR, and related techniques.

You will independently plan experiments, troubleshoot, and maintain rigorous electronic lab notebook records while contributing to publications and internal reviews.

Qualifications

• 2–3 years of work experience in an academic institute or pharmaceutical industry.
• Experience in immunology and molecular biology; purification and handling primary human lymphocytes desirable.
• Basic understanding of high-parameter multi-color flow cytometry desirable.

Responsibilities

• Develop immune functional cell-based assays using immunology research-related technologies (e.g. primary lymphocytes from human, monkey, and mouse, cytokine assays, multi-color flow cytometry).
• Screen and analyze data for protein or small molecule drugs in cell-based assays.
• Isolate, purify, and culture human, monkey, and mouse lymphocytes for in vitro assays.
• Develop cellular activity assays using techniques such as western blot, qPCR, intracellular staining, phospho flow cytometry, confocal/imaging related techniques, ligand-binding assays like ELISA and MSD multiplex.
• Investigate cellular signal transduction including cytokine/receptor signaling.
• Perform and evaluate functional effects of siRNA mediated gene knockdown and transfection/transduction mediated gene expression in cell lines and primary human cells.
• Independently design and execute experiments, troubleshoot, analyze, interpret and present experimental data.
• Adhere to good research practices and timely, thorough laboratory e-notebook maintenance.

Skills

Immunology

Molecular biology

Cell culture

qPCR

Western blot

Confocal imaging

ELISA

Flow cytometry

Data analysis

Gene knockdown

Education

B.S or M.S. in biology, molecular and cellular immunology

Tools

Flow cytometry

qPCR

Western blot

ELISA

Confocal imaging

Job description

Eli Lilly and Company in Indianapolis, IN, is seeking a scientist to design and execute immunology cell-based assays and analyze preclinical data supporting novel therapeutics. You will work with primary human, monkey, and mouse lymphocytes, applying multi-parameter flow cytometry, qPCR, and related techniques.

You will independently plan experiments, troubleshoot, and maintain rigorous electronic lab notebook records while contributing to publications and internal reviews.