Flow Cytometry CoreManager

Integrated Resources, Inc ( IRI ); Cambridge (MA); On-site; USD 120,000 - 180,000; Full time

Job summary

Integrated Resources, Inc is seeking a Flow Cytometry Core Manager at the Kendall Square site to oversee a suite of instruments including BD Symphony, BD FACSLyric, Attune CytPix, Opteon spectral platforms, and cell sorters. This contract role combines scientific leadership with hands-on instrument ownership and proactive user support.

You will manage daily operations, training, reagent inventory, data analysis, and cross-functional collaboration, ensuring data quality and thorough documentation

Qualifications

• Demonstrated expertise in multiparameter panel design, antibody titration, compensation, and data analysis (FlowJo required).
• Experience operating cell sorters (e.g., Sony SH800S or equivalent).
• Experience with spectral flow cytometry platforms (e.g., NovoCyte Opteon or equivalent).

Responsibilities

• Operate and maintain six flow cytometry platforms; perform preventive maintenance and QC procedures.
• Train and onboard site users; provide expert guidance on panel design and staining protocols.
• Support sample workflow, acquisition, data analysis, and reagent inventory; coordinate vendor support.

Skills

Multiparameter panel design

Antibody titration

Compensation

Data analysis

FlowJo

GraphPad Prism

Education

Master's degree in a related field

Bachelor's degree in a related field

Tools

Sony SH800S

NovoCyte Opteon

ThermoFisher Attune CytPix

BD FACSLyric

FACSDiva

Benchling

GraphPad Prism

Job description

• The Flow Cytometry Core Manager is a contract position serving as the primary scientific and operational lead for the flow cytometry core at the Kendall Square site, overseeing a suite of instruments spanning conventional, spectral, imaging, and cell sorting platforms.
• This individual is responsible for the daily operation, preventive maintenance, and troubleshooting of all flow cytometry instrumentation, as well as providing expert-level technical guidance tinternal users across research teams.
• This role will support research programs across cross-functional teams. The successful candidate will combine deep technical expertise in flow cytometry with strong organizational skills and a service-oriented mindset, balancing hands‑on instrument ownership with proactive user support, rigorous record‑keeping, and effective vendor management.

Key Responsibilities:

Instrument Operations & Maintenance:

• Perform daily startup and shutdown procedures for all instruments (BD Symphony, BD FACSLyric, ThermFisher Attune CytPix, Opteon, Guava, and Sony cell sorters) in accordance with manufacturer specifications and site SOPs; execute daily and weekly preventive maintenance routines, including CST checks, fluidics maintenance, and deep-cleaning protocols tensure instrument performance and data quality.
• Serve as the primary emergency contact and first responder for all flow cytometry instrument issues across the Kendall Square site; act as the primary liaison with manufacturer customer support teams (Sony, Agilent/NovoCyte, BD, ThermFisher, Guava easyCyte) for service requests, escalations, and repair coordination; apply advanced technical expertise tindependently troubleshoot instrument, reagent, and assay-related issues as they arise.

User Training & Onboarding:

• Serve as the principal trainer for all site flow cytometry users; develop and deliver onboarding training for new users across all platforms; provide guidance scaled tusers ranging from novice texperienced scientists.
• Provide on-demand expert guidance on panel design (10+ color), antibody titration, fluorochrome selection, and staining protocols tailored tusers' experimental needs; assist with compensation setup and spectral unmixing for medium- and large-scale multiparameter panels.

Sample Workflow Support:

• Support ex vivprocessing and acquisition of mouse, human PBMC, and NHP samples on core instrumentation

Sample Acquisition Support:

• Run samples on behalf of users as needed and provide hands‑on flow staining support tensure experimental success across the site.

Data Analysis Support:

• Perform or assist with data analysis on an occasional basis, including gating strategy review and FlowJworkflow guidance.

Reagent Inventory & Digital Record-Keeping:

• Maintain a well‑organized, adequately stocked inventory of all flow cytometry reagents, including antibodies, viability dyes, buffers, and calibration beads; manage Benchling inventory records, ensuring accurate and up‑to‑date entries for all staining reagents, lot numbers, and expiration dates; monitor freezer stocks and coordinate timely reagent reordering tprevent stockouts and ensure continuity of research operations.

Cross‑functional collaboration:

• Interface with partner teams (e.g. Immunology, Translational Biology, Analytical Development) tcoordinate experiments, share data, and provide technical consultation.

Team Structure – how does this role fit with the team The [EG-100] Scientist operates with a high degree of autonomy as the sole owner of site‑wide flow cytometry operations.

This individual is expected to:

• Own all aspects of instrument uptime, performance, and maintenance across six platforms with minimal day‑to‑day supervision
• Serve as the recognized site expert and primary escalation point for all flow cytometry‑related questions and emergencies
• Proactively identify gaps in reagent inventory, instrument performance, or user capability and address them before they impact research
• Build and maintain productive relationships with internal users and external vendor contacts
• Contribute tcontinuous improvement of site workflows, training materials, and operational procedures
• This is a high‑autonomy, high‑accountability role requiring both deep technical expertise and a strong service and operational mindset.

Role in Team

Must‑have Qualifications

• Demonstrated expertise in multiparameter panel design, antibody titration, compensation, and data analysis (FlowJrequired)
• Experience operating cell sorters (e.g., Sony SH800S or equivalent)
• Experience with spectral flow cytometry platforms (e.g., NovoCyte Opteon or equivalent)

Familiarity with at least three of the following instruments:

• Sony SH800S Cell Sorter
• NovoCyte Opteon Spectral Flow Cytometer
• ThermFisher Attune CytPix
• BD FACSLyric
• Experience performing and documenting preventive maintenance, CST checks, and instrument QC procedures
• Proficiency in FlowJand GraphPad Prism for data visualization and analysis

Nice‑to‑have Qualifications:

• Experience in a shared core facility or multi‑user laboratory environment
• Familiarity with spectral unmixing workflows and instrument interface software (e.g., FACSDiva)
• Experience with GLP‑adjacent or IND‑enabling study environments
• Experience with ex vivtissue processing (e.g. blood, spleen, lymph node etc.), whole blood, and human PBMCs
• Exposure tprimary immune cell biology (T cells, PBMCs, NHP samples) tcontextualize user support
• Experience coordinating with external vendors and service engineers
• Proficiency in Benchling for inventory management and ELN documentation

Technical Skills

• Non‑Technical Skills Strong organizational skills and the ability tmanage reagent inventory, instrument scheduling, and user queues concurrently
• Excellent interpersonal and communication skills; demonstrated ability ttrain and support scientists at varying levels of expertise

Additional Standout Experience

• Required Experience 3+ years with M.S.; 5+ years with B.S.