Flow Cytometry Core Manager

Integrated Resources, Inc ( IRI ); Cambridge (MA); On-site; USD 90,000 - 140,000; Full time

Job summary

Integrated Resources, Inc. (IRI) in Cambridge, MA seeks a Flow Cytometry Core Manager for a contract role to lead the Kendall Square core.

You will oversee instrument operations across BD Symphony, FACSLyric, Attune CytPix, NovoCyte Opteon, Sony sorters, and more, ensuring data quality and uptime. Responsibilities include daily maintenance, user training, panel design guidance, sample processing, data analysis, inventory control, and vendor coordination, with strong emphasis on collaboration and

Qualifications

• Demonstrated expertise in multiparameter panel design and data analysis.
• Experience with antibody titration and compensation.
• Knowledge of FlowJo and spectral unmixing.
• Experience with cell sorters and spectral flow cytometry platforms.

Responsibilities

• Oversee daily operations and maintenance of flow cytometry instruments.
• Train and onboard site users.
• Provide expert guidance on panel design and staining protocols.
• Coordinate vendor service and instrument repairs.
• Maintain reagent inventory and ELN records.

Skills

Panel design

Antibody titration

Compensation

Data analysis

Training

Instrument troubleshooting

Education

MS in relevant science

BS in relevant science

Tools

FlowJo

GraphPad Prism

Benchling

Sony SH800S

NovoCyte Opteon

BD FACSLyric

Job description

• The Flow Cytometry Core Manager is a contract position serving as the primary scientific and operational lead for the flow cytometry core at the Kendall Square site, overseeing a suite of instruments spanning conventional, spectral, imaging, and cell sorting platforms.
• This individual is responsible for the daily operation, preventive maintenance, and troubleshooting of all flow cytometry instrumentation, as well as providing expert-level technical guidance tinternal users across research teams.
• This role will support research programs across cross-functional teams. The successful candidate will combine deep technical expertise in flow cytometry with strong organizational skills and a service-oriented mindset, balancing hands‑on instrument ownership with proactive user support, rigorous record‑keeping, and effective vendor management.

Key Responsibilities:

Instrument Operations & Maintenance:

• Perform daily startup and shutdown procedures for all instruments (BD Symphony, BD FACSLyric, ThermFisher Attune CytPix, Opteon, Guava, and Sony cell sorters) in accordance with manufacturer specifications and site SOPs; execute daily and weekly preventive maintenance routines, including CST checks, fluidics maintenance, and deep-cleaning protocols tensure instrument performance and data quality.
• Serve as the primary emergency contact and first responder for all flow cytometry instrument issues across the Kendall Square site; act as the primary liaison with manufacturer customer support teams (Sony, Agilent/NovoCyte, BD, ThermFisher, Guava easyCyte) for service requests, escalations, and repair coordination; apply advanced technical expertise tindependently troubleshoot instrument, reagent, and assay-related issues as they arise.

User Training & Onboarding:

• Serve as the principal trainer for all site flow cytometry users; develop and deliver onboarding training for new users across all platforms; provide guidance scaled tusers ranging from novice texperienced scientists.
• Provide on-demand expert guidance on panel design (10+ color), antibody titration, fluorochrome selection, and staining protocols tailored tusers' experimental needs; assist with compensation setup and spectral unmixing for medium- and large-scale multiparameter panels.

Sample Workflow Support:

• Support ex vivprocessing and acquisition of mouse, human PBMC, and NHP samples on core instrumentation

Sample Acquisition Support:

• Run samples on behalf of users as needed and provide hands‑on flow staining support tensure experimental success across the site.

Data Analysis Support:

• Perform or assist with data analysis on an occasional basis, including gating strategy review and FlowJworkflow guidance.

Reagent Inventory & Digital Record-Keeping:

• Maintain a well‑organized, adequately stocked inventory of all flow cytometry reagents, including antibodies, viability dyes, buffers, and calibration beads; manage Benchling inventory records, ensuring accurate and up‑to‑date entries for all staining reagents, lot numbers, and expiration dates; monitor freezer stocks and coordinate timely reagent reordering tprevent stockouts and ensure continuity of research operations.

Cross‑functional collaboration:

• Interface with partner teams (e.g. Immunology, Translational Biology, Analytical Development) tcoordinate experiments, share data, and provide technical consultation.

Team Structure – how does this role fit with the team The [EG-100] Scientist operates with a high degree of autonomy as the sole owner of site‑wide flow cytometry operations.

This individual is expected to:

• Own all aspects of instrument uptime, performance, and maintenance across six platforms with minimal day‑to‑day supervision
• Serve as the recognized site expert and primary escalation point for all flow cytometry‑related questions and emergencies
• Proactively identify gaps in reagent inventory, instrument performance, or user capability and address them before they impact research
• Build and maintain productive relationships with internal users and external vendor contacts
• Contribute tcontinuous improvement of site workflows, training materials, and operational procedures
• This is a high‑autonomy, high‑accountability role requiring both deep technical expertise and a strong service and operational mindset.

Role in Team

Must‑have Qualifications

• Demonstrated expertise in multiparameter panel design, antibody titration, compensation, and data analysis (FlowJrequired)
• Experience operating cell sorters (e.g., Sony SH800S or equivalent)
• Experience with spectral flow cytometry platforms (e.g., NovoCyte Opteon or equivalent)

Familiarity with at least three of the following instruments:

• Sony SH800S Cell Sorter
• NovoCyte Opteon Spectral Flow Cytometer
• ThermFisher Attune CytPix
• BD FACSLyric
• Experience performing and documenting preventive maintenance, CST checks, and instrument QC procedures
• Proficiency in FlowJand GraphPad Prism for data visualization and analysis

Nice‑to‑have Qualifications:

• Experience in a shared core facility or multi‑user laboratory environment
• Familiarity with spectral unmixing workflows and instrument interface software (e.g., FACSDiva)
• Experience with GLP‑adjacent or IND‑enabling study environments
• Experience with ex vivtissue processing (e.g. blood, spleen, lymph node etc.), whole blood, and human PBMCs
• Exposure tprimary immune cell biology (T cells, PBMCs, NHP samples) tcontextualize user support
• Experience coordinating with external vendors and service engineers
• Proficiency in Benchling for inventory management and ELN documentation

Technical Skills

• Non‑Technical Skills Strong organizational skills and the ability tmanage reagent inventory, instrument scheduling, and user queues concurrently
• Excellent interpersonal and communication skills; demonstrated ability ttrain and support scientists at varying levels of expertise

Additional Standout Experience

• Required Experience 3+ years with M.S.; 5+ years with B.S.