Flow Cytometry Specialist, Manager # 26-24159

Us Tech Solutions; Cambridge (MA); On-site; USD 120,000 - 160,000; Full time

Job summary

US Tech Solutions is seeking a Flow Cytometry Core Manager for our Kendall Square site. You will oversee daily operation, PM, and troubleshooting across six flow cytometry platforms, providing expert guidance to researchers and managing vendor relations.

The role combines hands-on instrument ownership with proactive user support, rigorous record-keeping, and collaboration with cross-functional teams. A strong background in multiparameter panel design and data analysis is required.

Qualifications

• Demonstrated expertise in multiparameter panel design, antibody titration, compensation, and data analysis (FlowJo).
• Experience operating cell sorters (Sony SH800S or equivalent).
• Experience with spectral flow cytometry platforms (NovoCyte Opteon or equivalent).
• Familiarity with at least three of listed instruments (SH800S, Opteon, FACSymphony A3, Attune CytPix, FACSLyric).
• Experience performing preventive maintenance, CST checks, and instrument QC procedures.
• Proficiency in FlowJo and GraphPad Prism for data visualization and analysis.

Responsibilities

• Perform daily startup/shutdown procedures for all instruments per manufacturer specs and SOPs.
• Troubleshoot instruments and coordinate with manufacturer support and service teams.
• Train and onboard site users; provide guidance across varying experience levels.
• Assist with panel design, staining protocols, and spectral unmixing for large panels.
• Support ex vivo processing and acquisition of mouse, human PBMC, and NHP samples.
• Run samples for users and provide hands-on flow staining support as needed.
• Assist with data analysis, gating strategies, and FlowJo workflows.
• Maintain organized reagent inventory and Benchling records; monitor freezer stocks.

Skills

Panel design

Antibody titration

Compensation

FlowJo

Spectral flow cytometry

Cell sorting

Instrumentation troubleshooting

Vendor liaison

Training & onboarding

Data analysis

Inventory management

Education

Bachelor's degree in life sciences

Master's degree preferred

Tools

Sony SH800S

NovoCyte Opteon

BD FACSymphony A3

Thermo Fisher Attune CytPix

BD FACSLyric

Guava easyCyte

Job description

Duration: 12 months (with extension possibility) Working Hours: 9-5 PM

Job Description

The Flow Cytometry Core Manager is a contract position serving as the primary scientific and operational lead for the flow cytometry core at the Kendall Square site, overseeing a suite of instruments spanning conventional, spectral, imaging, and cell sorting platforms. This individual is responsible for the daily operation, preventive maintenance, and troubleshooting of all flow cytometry instrumentation, as well as providing expert-level technical guidance to internal users across research teams. This role will support research programs across cross-functional teams. The successful candidate will combine deep technical expertise in flow cytometry with strong organizational skills and a service-oriented mindset, balancing hands‑on instrument ownership with proactive user support, rigorous record‑keeping, and effective vendor management.

Key Responsibilities

• Perform daily startup and shutdown procedures for all instruments (Client Symphony, Client FACSLyric, Thermo Fisher Attune CytPix, Opteon, Guava, and Sony cell sorters) in accordance with manufacturer specifications and site SOPs; execute daily and weekly preventive maintenance routines, including CST checks, fluidics maintenance, and deep-cleaning protocols to ensure instrument performance and data quality.
• Instrument Troubleshooting & Vendor Liaison: Serve as the primary emergency contact and first responder for all flow cytometry instrument issues across the Kendall Square site; act as the primary liaison with manufacturer customer support teams (Sony, Agilent/NovoCyte, Client, Thermo Fisher, Guava easyCyte) for service requests, escalations, and repair coordination; apply advanced technical expertise to independently troubleshoot instrument, reagent, and assay-related issues as they arise.
• User Training & Onboarding: Serve as the principal trainer for all site flow cytometry users; develop and deliver onboarding training for new users across all platforms; provide guidance scaled to users ranging from novice to experienced scientists.
• Panel Design & Staining Support: Provide on-demand expert guidance on panel design (10+ color), antibody titration, fluorochrome selection, and staining protocols tailored to users' experimental needs; assist with compensation setup and spectral unmixing for medium- and large-scale multiparameter panels.
• Sample Workflow Support: Support ex vivo processing and acquisition of mouse, human PBMC, and NHP samples on core instrumentation
• Sample Acquisition Support: Run samples on behalf of users as needed and provide hands-on flow staining support to ensure experimental success across the site.
• Data Analysis Support: Perform or assist with data analysis on an occasional basis, including gating strategy review and FlowJo workflow guidance.
• Reagent Inventory & Digital Record-Keeping: Maintain a well-organized, adequately stocked inventory of all flow cytometry reagents, including antibodies, viability dyes, buffers, and calibration beads; manage Benchling inventory records, ensuring accurate and up-to-date entries for all staining reagents, lot numbers, and expiration dates; monitor freezer stocks and coordinate timely reagent reordering to prevent stockouts and ensure continuity of research operations.
• Cross-functional collaboration: Interface with partner teams (e.g. Immunology, Translational Biology, Analytical Development) to coordinate experiments, share data, and provide technical consultation Team Structure - how does this role fit with the teamThe [EG-100] Scientist operates with a high degree of autonomy as the sole owner of site-wide flow cytometry operations. This individual is expected to:
• Own all aspects of instrument uptime, performance, and maintenance across six platforms with minimal day-to-day supervision
• Serve as the recognized site expert and primary escalation point for all flow cytometry-related questions and emergencies
• Proactively identify gaps in reagent inventory, instrument performance, or user capability and address them before they impact research
• Build and maintain productive relationships with internal users and external vendor contacts
• Contribute to continuous improvement of site workflows, training materials, and operational procedures

Must-have Qualifications

• Demonstrated expertise in multiparameter panel design, antibody titration, compensation, and data analysis (FlowJo required)
• Experience operating cell sorters (e.g., Sony SH800S or equivalent)
• Experience with spectral flow cytometry platforms (e.g., NovoCyte Opteon or equivalent)
• Familiarity with at least three of the following instruments:
• oSony SH800S Cell Sorter
• oNovoCyte Opteon Spectral Flow Cytometer
• oBD FACSymphony A3
• oThermo Fisher Attune CytPix
• oBD FACSLyric
• Experience performing and documenting preventive maintenance, CST checks, and instrument QC procedures
• Proficiency in FlowJo and GraphPad Prism for data visualization and analysis

Nice-to-have Qualifications

• Experience in a shared core facility or multi-user laboratory environment
• Familiarity with spectral unmixing workflows and instrument interface software (e.g., FACSDiva)
• Experience with GLP-adjacent or IND-enabling study environments
• Experience with ex vivo tissue processing (e.g. blood, spleen, lymph node etc.), whole blood, and human PBMCs
• Exposure to primary immune cell biology (T cells, PBMCs, NHP samples) to contextualize user support
• Experience coordinating with external vendors and service engineers
• Proficiency in Benchling for inventory management and ELN documentation

Required Experience

• 3+ years with M.S.; 5+ years with B.S. (Preferred Experience3+ years with M.S)

About US Tech Solutions

US Tech Solutions is a global staff augmentation firm providing a wide range of talent on-demand and total workforce solutions. To know more about US Tech Solutions, please visit www.ustechsolutions.com.

Equal Opportunity Employer

US Tech Solutions is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, colour, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.

AI Statement

By applying, you acknowledge that AI-assisted tools may be used during hiring.

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